EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.85 | 0.013 | - |
(2R,3S)-3-isopropylmalate | pH 7.6, 20°C, stopped flow experiment | Thermus thermophilus | |
1.1.1.85 | 0.25 | - |
NAD+ | pH 7.6, 20°C, stopped flow experiment | Thermus thermophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.85 | Thermus thermophilus | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.85 | (2R,3S)-3-isopropylmalate + NAD+ | - |
Thermus thermophilus | 2-oxoisocaproate + NADH + H+ + CO2 | - |
? | |
1.1.1.85 | additional information | reaction scheme based on incorporation of the rate constant of a fast protein conformational change such as domain closure derived from the separately recorded time-dependent formation of the fluorescence resonance energy transfer signal. The rate-limiting step seems to be another slower conformational change that allows product release | Thermus thermophilus | ? | - |
? |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.85 | 2.9 | - |
(2R,3S)-3-isopropylmalate | pH 7.6, 20°C, quenched flow experiment | Thermus thermophilus | |
1.1.1.85 | 3.4 | - |
(2R,3S)-3-isopropylmalate | pH 7.6, 20°C, stopped flow experiment | Thermus thermophilus |