BRENDA - Enzyme Database show

A non-canonical caleosin from Arabidopsis efficiently epoxidizes physiological unsaturated fatty acids with complete stereoselectivity

Blee, E.; Flenet, M.; Boachon, B.; Fauconnier, M.L.; FEBS J. 279, 3981-3995 (2012)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.11.2.3
expression in Saccharomyces cerevisiae
Arabidopsis thaliana
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.11.2.3
membrane
associated with membrane
Arabidopsis thaliana
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.11.2.3
Ca2+
required
Arabidopsis thaliana
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.11.2.3
24000
-
x * 24000, SDS-PAGE
Arabidopsis thaliana
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.11.2.3
linoleic acid + cumene hydroperoxide
Arabidopsis thaliana
-
?
64% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4
-
?
1.11.2.3
linolenic acid + cumene hydroperoxide
Arabidopsis thaliana
74% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4
linolenic acid 9,10-15,16-diepoxide + ?
major product, (R),(S)-epoxide enantiomers
-
?
1.11.2.3
additional information
Arabidopsis thaliana
enzyme is an efficient fatty acid epoxygenase, catalyzing the oxidation of cis double bonds of unsaturated fatty acids. The C-12,13 double bond of these unsaturated fatty acids is the least favoured. Isoform PXG4 catalyzes exclusively the formation of (R),(S)-epoxide enantiomers, which is the absolute stereochemistry of the epoxides found in planta. Poor substrate: oleic acid methyl ester
?
-
-
-
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.11.2.3
Arabidopsis thaliana
Q9CAB7
-
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.11.2.3
flower
expression in root, stem, leaf and flower
Arabidopsis thaliana
-
1.11.2.3
leaf
expression in root, stem, leaf and flower
Arabidopsis thaliana
-
1.11.2.3
root
expression in root, stem, leaf and flower
Arabidopsis thaliana
-
1.11.2.3
stem
expression in root, stem, leaf and flower
Arabidopsis thaliana
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.2.3
linoleic acid + cumene hydroperoxide
-
724973
Arabidopsis thaliana
?
64% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4
-
-
?
1.11.2.3
linolenic acid + cumene hydroperoxide
74% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4
724973
Arabidopsis thaliana
linolenic acid 9,10-15,16-diepoxide + ?
major product, (R),(S)-epoxide enantiomers
-
-
?
1.11.2.3
additional information
enzyme is an efficient fatty acid epoxygenase, catalyzing the oxidation of cis double bonds of unsaturated fatty acids. The C-12,13 double bond of these unsaturated fatty acids is the least favoured. Isoform PXG4 catalyzes exclusively the formation of (R),(S)-epoxide enantiomers, which is the absolute stereochemistry of the epoxides found in planta. Poor substrate: oleic acid methyl ester
724973
Arabidopsis thaliana
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.11.2.3
?
x * 24000, SDS-PAGE
Arabidopsis thaliana
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.11.2.3
heme
contains a protoheme of type b, with 0.6 nmol of heme per mg protein
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.11.2.3
expression in Saccharomyces cerevisiae
Arabidopsis thaliana
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.11.2.3
heme
contains a protoheme of type b, with 0.6 nmol of heme per mg protein
Arabidopsis thaliana
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.11.2.3
membrane
associated with membrane
Arabidopsis thaliana
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.11.2.3
Ca2+
required
Arabidopsis thaliana
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.11.2.3
24000
-
x * 24000, SDS-PAGE
Arabidopsis thaliana
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.11.2.3
linoleic acid + cumene hydroperoxide
Arabidopsis thaliana
-
?
64% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4
-
?
1.11.2.3
linolenic acid + cumene hydroperoxide
Arabidopsis thaliana
74% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4
linolenic acid 9,10-15,16-diepoxide + ?
major product, (R),(S)-epoxide enantiomers
-
?
1.11.2.3
additional information
Arabidopsis thaliana
enzyme is an efficient fatty acid epoxygenase, catalyzing the oxidation of cis double bonds of unsaturated fatty acids. The C-12,13 double bond of these unsaturated fatty acids is the least favoured. Isoform PXG4 catalyzes exclusively the formation of (R),(S)-epoxide enantiomers, which is the absolute stereochemistry of the epoxides found in planta. Poor substrate: oleic acid methyl ester
?
-
-
-
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.11.2.3
flower
expression in root, stem, leaf and flower
Arabidopsis thaliana
-
1.11.2.3
leaf
expression in root, stem, leaf and flower
Arabidopsis thaliana
-
1.11.2.3
root
expression in root, stem, leaf and flower
Arabidopsis thaliana
-
1.11.2.3
stem
expression in root, stem, leaf and flower
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.11.2.3
linoleic acid + cumene hydroperoxide
-
724973
Arabidopsis thaliana
?
64% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4
-
-
?
1.11.2.3
linolenic acid + cumene hydroperoxide
74% conversion, physiological linoleic and linolenic acids are the preferred substrates for isoform PXG4
724973
Arabidopsis thaliana
linolenic acid 9,10-15,16-diepoxide + ?
major product, (R),(S)-epoxide enantiomers
-
-
?
1.11.2.3
additional information
enzyme is an efficient fatty acid epoxygenase, catalyzing the oxidation of cis double bonds of unsaturated fatty acids. The C-12,13 double bond of these unsaturated fatty acids is the least favoured. Isoform PXG4 catalyzes exclusively the formation of (R),(S)-epoxide enantiomers, which is the absolute stereochemistry of the epoxides found in planta. Poor substrate: oleic acid methyl ester
724973
Arabidopsis thaliana
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.11.2.3
?
x * 24000, SDS-PAGE
Arabidopsis thaliana