EC Number | Cloned (Comment) | Organism |
---|---|---|
1.4.3.4 | expressed in Pichia pastoris | Homo sapiens |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
1.4.3.4 | using 12% (w/v) polyethylene glycol 4000, 100 mM N-(2-acetamido)-2-iminodiacetic acid buffer pH 6.5, and 70 mM lithium sulfate | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.4.3.4 | I199A | the mutant exhibits catalytic properties with 4fold increased amine Km but unaltered kcat values | Homo sapiens |
1.4.3.4 | I199A/Y326A | the mutant exhibits catalytic properties with 75fold increased amine Km but unaltered kcat values. The mutant shows inhibitor binding properties more similar to those of isoform MAO A than to isoform MAO B. Benzylamine is a poor substrate for the double mutant | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.4 | 1,4-diphenyl-1,3-butadiene | - |
Homo sapiens | |
1.4.3.4 | 1,4-diphenyl-2-butene | - |
Homo sapiens | |
1.4.3.4 | 8-(3-chloro)styrylcaffeine | - |
Homo sapiens | |
1.4.3.4 | farnesol | - |
Homo sapiens | |
1.4.3.4 | Harmane | - |
Homo sapiens | |
1.4.3.4 | isatin | non-specific reversible inhibitor | Homo sapiens | |
1.4.3.4 | methylene blue | - |
Homo sapiens | |
1.4.3.4 | additional information | isoform MAO B is not inhibited by pirlindole | Homo sapiens | |
1.4.3.4 | safinamide | - |
Homo sapiens | |
1.4.3.4 | tranylcypromine | - |
Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.4 | 0.0089 | - |
phenylbutylamine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.0094 | - |
Phenylethylamine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.019 | - |
phenylbutylamine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.027 | - |
kynuramine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.04 | - |
Phenylethylamine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.055 | - |
phenylbutylamine | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.094 | - |
kynuramine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.15 | - |
benzylamine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.289 | - |
kynuramine | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.703 | - |
Phenylethylamine | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 2.133 | - |
benzylamine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.4.3.4 | Homo sapiens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.4.3.4 | - |
Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.4.3.4 | liver | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.4.3.4 | 1-[4-(trifluoromethyl)phenyl]methanamine + H2O + O2 | - |
Homo sapiens | 4-(trifluoromethyl)benzaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.4 | benzylamine + H2O + O2 | - |
Homo sapiens | benzaldehyde + NH3 + H2O2 | - |
? | |
1.4.3.4 | kynuramine + H2O + O2 | - |
Homo sapiens | 3-(2-aminophenyl)-3-oxopropanal + NH3 + H2O2 | - |
? | |
1.4.3.4 | phenylbutylamine + H2O + O2 | - |
Homo sapiens | 2-phenylbutanal + NH3 + H2O2 + NH3 + H2O2 | - |
? | |
1.4.3.4 | phenylethylamine + H2O + O2 | - |
Homo sapiens | 2-phenylethanal + NH3 + H2O2 + NH3 + H2O2 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.4.3.4 | MAO B | isoform | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.4 | 1.07 | - |
kynuramine | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 1.47 | - |
kynuramine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 1.6 | - |
kynuramine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 1.83 | - |
Phenylethylamine | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 1.83 | - |
phenylbutylamine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 2.05 | - |
Phenylethylamine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 2.25 | - |
phenylbutylamine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 2.34 | - |
phenylbutylamine | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 2.87 | - |
Phenylethylamine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 3.8 | - |
benzylamine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 5 | - |
benzylamine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.4.3.4 | FAD | - |
Homo sapiens |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.4 | 0.000021 | - |
safinamide | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.000143 | - |
methylene blue | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.000214 | - |
methylene blue | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.00027 | - |
8-(3-chloro)styrylcaffeine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.0005 | - |
safinamide | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.001 | - |
1,4-diphenyl-1,3-butadiene | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.00101 | - |
methylene blue | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.0017 | - |
8-(3-chloro)styrylcaffeine | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.0023 | - |
farnesol | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.0027 | - |
farnesol | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.003 | - |
isatin | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.004 | - |
safinamide | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.007 | - |
1,4-diphenyl-1,3-butadiene | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.0081 | - |
1,4-diphenyl-2-butene | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.011 | - |
tranylcypromine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.012 | - |
isatin | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.016 | - |
tranylcypromine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.017 | - |
tranylcypromine | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.018 | - |
farnesol | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.0345 | - |
1,4-diphenyl-2-butene | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.14 | - |
Harmane | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.165 | - |
Harmane | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.36 | - |
isatin | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 0.55 | - |
Harmane | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.4.3.4 | 1.78 | - |
benzylamine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 2.7 | - |
Phenylethylamine | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 3.7 | - |
kynuramine | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 15.6 | - |
kynuramine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 33.3 | - |
benzylamine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 43.3 | - |
phenylbutylamine | mutant enzyme I199A/Y326A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 51.7 | - |
Phenylethylamine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 59.2 | - |
kynuramine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 96.7 | - |
phenylbutylamine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 253.3 | - |
phenylbutylamine | mutant enzyme I199A, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens | |
1.4.3.4 | 300 | - |
Phenylethylamine | wild type isoform MAO B, at 25°C in 50 mM phosphate buffer (pH 7.5) | Homo sapiens |