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Literature summary extracted from

  • Frattini, L.F.; Piubelli, L.; Sacchi, S.; Molla, G.; Pollegioni, L.
    Is rat an appropriate animal model to study the involvement of D-serine catabolism in schizophrenia? Insights from characterization of D-amino acid oxidase (2011), FEBS J., 278, 4362-4373.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.3 expressed in Escherichia coli BL21(DE3)Star cells Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.3 6-chlorobenzo(d)isoxazol-3-ol
-
 Rattus norvegicus
1.4.3.3 anthranilate
-
 Rattus norvegicus
1.4.3.3 benzoate
-
 Rattus norvegicus
1.4.3.3 chlorpromazine FAD-competitive inhibitor Rattus norvegicus
1.4.3.3 crotonate
-
 Rattus norvegicus
1.4.3.3 sulfite
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.3 0.5
-
 O2 in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C Rattus norvegicus
1.4.3.3 15
-
 D-tryptophan in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C Rattus norvegicus
1.4.3.3 35
-
 D-phenylalanine in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C Rattus norvegicus
1.4.3.3 86
-
 D-proline in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C Rattus norvegicus
1.4.3.3 140
-
 D-alanine in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C Rattus norvegicus
1.4.3.3 310
-
 D-serine in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.3 40000
-
 1 * 40000, SDS-PAGE Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.3 D-amino acid + H2O + O2 Rattus norvegicus
-
 2-oxocarboxylate + NH3 + H2O2
-
 ?
1.4.3.3 additional information Rattus norvegicus the enzyme interacts with the mammalian protein modulator pLG72 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.3 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.3 Ni2+-chelate affinity column chromatography Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.3 kidney
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.3 D-alanine + H2O + O2
-
 Rattus norvegicus pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-amino acid + H2O + O2
-
 Rattus norvegicus 2-oxocarboxylate + NH3 + H2O2
-
 ?
1.4.3.3 D-phenylalanine + H2O + O2
-
 Rattus norvegicus phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.3 D-proline + H2O + O2
-
 Rattus norvegicus 2-oxopentanoic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-serine + H2O + O2
-
 Rattus norvegicus 2-oxo-3-hydroxypropionic acid + NH3 + H2O2
-
 ?
1.4.3.3 D-tryptophan + H2O + O2
-
 Rattus norvegicus indol-3-pyruvate + NH3 + H2O2
-
 ?
1.4.3.3 additional information the enzyme interacts with the mammalian protein modulator pLG72 Rattus norvegicus ?
-
 ?
1.4.3.3 additional information D-aspartate and glycine are no substrates Rattus norvegicus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.3 monomer 1 * 40000, SDS-PAGE Rattus norvegicus

Synonyms

EC Number Synonyms Comment Organism
1.4.3.3 D-amino acid oxidase
-
 Rattus norvegicus
1.4.3.3 DAAO
-
 Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.3 3.7
-
 D-tryptophan in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C Rattus norvegicus
1.4.3.3 6.4
-
 D-serine in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C Rattus norvegicus
1.4.3.3 8.5
-
 D-phenylalanine in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C Rattus norvegicus
1.4.3.3 27
-
 D-alanine in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C Rattus norvegicus
1.4.3.3 40
-
 O2 in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C Rattus norvegicus
1.4.3.3 47
-
 D-proline in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.3 FAD
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.4.3.3 0.4
-
 benzoate in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C Rattus norvegicus
1.4.3.3 1.6
-
 anthranilate in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C Rattus norvegicus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.3.3 0.02
-
 D-serine in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C Rattus norvegicus
1.4.3.3 0.2
-
 D-alanine in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C Rattus norvegicus
1.4.3.3 0.24
-
 D-phenylalanine in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C Rattus norvegicus
1.4.3.3 0.25
-
 D-tryptophan in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C Rattus norvegicus
1.4.3.3 0.55
-
 D-proline in 75 mM sodium diphosphate buffer (pH 8.3) at 25°C Rattus norvegicus