Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Welte, C.; Deppenmeier, U.
    Re-evaluation of the function of the F420 dehydrogenase in electron transport of Methanosarcina mazei (2011), FEBS J., 278, 1277-1287.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.7.2 expression in Escherichia coli DH5alpha Methanosarcina mazei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.7.2 0.0005
-
 reduced ferredoxin pH 7.0, temperature not specified in the publication Methanosarcina mazei
1.5.7.2 0.002
-
 oxidized coenzyme F420 pH 7.0, temperature not specified in the publication Methanosarcina mazei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.7.2 cytoplasm as a soluble single subunit it is involved in the reoxidation of reduced ferredoxin in the cytoplasm, whereby reduced F420 is produced that is then reoxidized by the complete Fpo complex Methanosarcina mazei 5737
-
1.5.7.2 membrane when the Fpo protein is connected to the membrane integral Fpo complex, it functions as an electron input module of the Fpo complex Methanosarcina mazei 16020
-
1.5.98.3 membrane
-
 Methanosarcina mazei 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.7.2 Iron-sulfur cluster presence of two FeS clusters, 8.7 mol of nonheme iron and 5.1 mol of acid-labile sulfur per mol of protein Methanosarcina mazei

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.7.2 oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ Methanosarcina mazei FpoF can function as an ferredoxin:F420 oxidoreductase when reduced ferredoxin accumulates in the cytoplasm. As a soluble single subunit it is involved in the reoxidation of reduced ferredoxin in the cytoplasm, whereby reduced F420 is produced that is then reoxidized by the complete Fpo complex reduced coenzyme F420 + 2 oxidized ferredoxin
-
 r
1.5.7.2 oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ Methanosarcina mazei DSM 3647 FpoF can function as an ferredoxin:F420 oxidoreductase when reduced ferredoxin accumulates in the cytoplasm. As a soluble single subunit it is involved in the reoxidation of reduced ferredoxin in the cytoplasm, whereby reduced F420 is produced that is then reoxidized by the complete Fpo complex reduced coenzyme F420 + 2 oxidized ferredoxin
-
 r
1.5.7.2 reduced coenzyme F420 + 2 oxidized ferredoxin Methanosarcina mazei when the Fpo protein is connected to the membrane integral Fpo complex, it functions as an electron input module of the Fpo complex oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+
-
 r
1.5.7.2 reduced coenzyme F420 + 2 oxidized ferredoxin Methanosarcina mazei DSM 3647 when the Fpo protein is connected to the membrane integral Fpo complex, it functions as an electron input module of the Fpo complex oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+
-
 r
1.5.98.3 reduced coenzyme F420 + methanophenazine Methanosarcina mazei
-
 oxidized coenzyme F420 + dihydromethanophenazine
-
 ?
1.5.98.3 reduced coenzyme F420 + methanophenazine Methanosarcina mazei DSM 7222
-
 oxidized coenzyme F420 + dihydromethanophenazine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.7.2 Methanosarcina mazei Q8PZ67
-
-
1.5.7.2 Methanosarcina mazei DSM 3647 Q8PZ67
-
-
1.5.98.3 Methanosarcina mazei
-
-
-
1.5.98.3 Methanosarcina mazei DSM 7222
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.7.2
-
 Methanosarcina mazei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.7.2 oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ FpoF can function as an ferredoxin:F420 oxidoreductase when reduced ferredoxin accumulates in the cytoplasm. As a soluble single subunit it is involved in the reoxidation of reduced ferredoxin in the cytoplasm, whereby reduced F420 is produced that is then reoxidized by the complete Fpo complex Methanosarcina mazei reduced coenzyme F420 + 2 oxidized ferredoxin
-
 r
1.5.7.2 oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+
-
 Methanosarcina mazei reduced coenzyme F420 + 2 oxidized ferredoxin
-
 r
1.5.7.2 oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+ FpoF can function as an ferredoxin:F420 oxidoreductase when reduced ferredoxin accumulates in the cytoplasm. As a soluble single subunit it is involved in the reoxidation of reduced ferredoxin in the cytoplasm, whereby reduced F420 is produced that is then reoxidized by the complete Fpo complex Methanosarcina mazei DSM 3647 reduced coenzyme F420 + 2 oxidized ferredoxin
-
 r
1.5.7.2 oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+
-
 Methanosarcina mazei DSM 3647 reduced coenzyme F420 + 2 oxidized ferredoxin
-
 r
1.5.7.2 reduced coenzyme F420 + 2 oxidized ferredoxin when the Fpo protein is connected to the membrane integral Fpo complex, it functions as an electron input module of the Fpo complex Methanosarcina mazei oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+
-
 r
1.5.7.2 reduced coenzyme F420 + 2 oxidized ferredoxin when the Fpo protein is connected to the membrane integral Fpo complex, it functions as an electron input module of the Fpo complex Methanosarcina mazei DSM 3647 oxidized coenzyme F420 + 2 reduced ferredoxin + 2 H+
-
 r
1.5.98.3 oxidized coenzyme F420 + reduced ferredoxin
-
 Methanosarcina mazei reduced coenzyme F420 + oxidized ferredoxin
-
 ?
1.5.98.3 oxidized coenzyme F420 + reduced ferredoxin
-
 Methanosarcina mazei DSM 7222 reduced coenzyme F420 + oxidized ferredoxin
-
 ?
1.5.98.3 reduced coenzyme F420 + methanophenazine
-
 Methanosarcina mazei oxidized coenzyme F420 + dihydromethanophenazine
-
 ?
1.5.98.3 reduced coenzyme F420 + methanophenazine
-
 Methanosarcina mazei DSM 7222 oxidized coenzyme F420 + dihydromethanophenazine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.7.2 Fd:F420 oxidoreductase
-
 Methanosarcina mazei
1.5.7.2 FpoF protein
-
 Methanosarcina mazei
1.5.7.2 mm0627
-
 Methanosarcina mazei
1.5.98.3 F420H2 dehydrogenase
-
 Methanosarcina mazei
1.5.98.3 Fpo
-
 Methanosarcina mazei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.7.2 7
-
 assay at Methanosarcina mazei

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.7.2 FAD presence of 1.0 mol FAD per mol protein Methanosarcina mazei

General Information

EC Number General Information Comment Organism
1.5.98.3 malfunction knockout mutants of the membrane-bound F420 dehydrogenase exhibit severe growth deficiencies with trimethylamine, but not with acetate, as substrates Methanosarcina mazei
1.5.98.3 metabolism the enzyme plays a major role in the oxidative part of methylotrophic methanogenesis Methanosarcina mazei