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Literature summary extracted from

  • Seedorf, H.; Hagemeier, C.H.; Shima, S.; Thauer, R.K.; Warkentin, E.; Ermler, U.
    Structure of coenzyme F420H2 oxidase (FprA), a di-iron flavoprotein from methanogenic archaea catalyzing the reduction of O2 to H2O (2007), FEBS J., 274, 1588-1599.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.22 overexpression in Escherichia coli Methanothermobacter marburgensis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.5.3.22 hanging and sitting drop vapor-diffusion method, crystal structures of F420H2 oxidase in a reduced state (2.25 A) and two oxidized states (1.7 A and 2.25 A) Methanothermobacter marburgensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.5.3.22 Fe2+ di-iron center, in the active reduced state [Fe(II)Fe(II)FMNH2], the two irons are surrounded by four histidines, one aspartate, one glutamate and one bridging aspartate Methanothermobacter marburgensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.22 2 reduced coenzyme F420 + O2 Methanothermobacter marburgensis
-
 2 oxidized coenzyme F420 + 2 H2O
-
 ?
1.5.3.22 2 reduced coenzyme F420 + O2 Methanothermobacter marburgensis DSM 2133
-
 2 oxidized coenzyme F420 + 2 H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.22 Methanothermobacter marburgensis Q50497
-
-
1.5.3.22 Methanothermobacter marburgensis DSM 2133 Q50497
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.22
-
 Methanothermobacter marburgensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.22 2 reduced coenzyme F420 + O2
-
 Methanothermobacter marburgensis 2 oxidized coenzyme F420 + 2 H2O
-
 ?
1.5.3.22 2 reduced coenzyme F420 + O2 the F420H2 oxidase reaction represents a ping-pong process where, in a first reaction, four electrons from the diferrous di-iron and FMNH2 are transferred to the dioxygen, thereby forming two water molecules without the release of reactive oxygen species, and in a second reaction, the two redox centers are re-reduced by two hydride transfer reactions between reduced coenzyme F420 and FMN Methanothermobacter marburgensis 2 oxidized coenzyme F420 + 2 H2O
-
 ?
1.5.3.22 2 reduced coenzyme F420 + O2
-
 Methanothermobacter marburgensis DSM 2133 2 oxidized coenzyme F420 + 2 H2O
-
 ?
1.5.3.22 2 reduced coenzyme F420 + O2 the F420H2 oxidase reaction represents a ping-pong process where, in a first reaction, four electrons from the diferrous di-iron and FMNH2 are transferred to the dioxygen, thereby forming two water molecules without the release of reactive oxygen species, and in a second reaction, the two redox centers are re-reduced by two hydride transfer reactions between reduced coenzyme F420 and FMN Methanothermobacter marburgensis DSM 2133 2 oxidized coenzyme F420 + 2 H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.3.22 FprA
-
 Methanothermobacter marburgensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.22 FMN the enzyme contains FMN Methanothermobacter marburgensis