EC Number | Cloned (Comment) | Organism |
---|---|---|
1.18.1.2 | expression in Escherichia coli | Sulfurisphaera tokodaii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.18.1.2 | 36600 | - |
2 * 36600, calculated from sequence | Sulfurisphaera tokodaii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.18.1.2 | 2 reduced ferredoxin + NADP+ | Sulfurisphaera tokodaii | the enzyme plays an important role in the redox cycle of ferredoxin in the archaeon | 2 oxidized ferredoxin + NADPH + H+ | - |
r | |
1.18.1.2 | 2 reduced ferredoxin + NADP+ | Sulfurisphaera tokodaii 7 | the enzyme plays an important role in the redox cycle of ferredoxin in the archaeon | 2 oxidized ferredoxin + NADPH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.18.1.2 | Sulfurisphaera tokodaii | Q96YN9 | - |
- |
1.18.1.2 | Sulfurisphaera tokodaii 7 | Q96YN9 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.18.1.2 | 2 reduced ferredoxin + NADP+ | the enzyme plays an important role in the redox cycle of ferredoxin in the archaeon | Sulfurisphaera tokodaii | 2 oxidized ferredoxin + NADPH + H+ | - |
r | |
1.18.1.2 | 2 reduced ferredoxin + NADP+ | high specificity for NADPH, the activity with NADH is hardly observed without the addition of an external flavin | Sulfurisphaera tokodaii | 2 oxidized ferredoxin + NADPH + H+ | - |
r | |
1.18.1.2 | 2 reduced ferredoxin + NADP+ | the enzyme plays an important role in the redox cycle of ferredoxin in the archaeon | Sulfurisphaera tokodaii 7 | 2 oxidized ferredoxin + NADPH + H+ | - |
r | |
1.18.1.2 | 2 reduced ferredoxin + NADP+ | high specificity for NADPH, the activity with NADH is hardly observed without the addition of an external flavin | Sulfurisphaera tokodaii 7 | 2 oxidized ferredoxin + NADPH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.18.1.2 | homodimer | 2 * 36600, calculated from sequence | Sulfurisphaera tokodaii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.18.1.2 | ST2133 | locus name | Sulfurisphaera tokodaii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.18.1.2 | 80 | - |
assay at | Sulfurisphaera tokodaii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.18.1.2 | 7.5 | - |
assay at | Sulfurisphaera tokodaii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.18.1.2 | FAD | contains 0.46 mol FAD/mol subunit | Sulfurisphaera tokodaii | |
1.18.1.2 | FMN | activates. Vmax is 1.38 and 21.8 U/mg (at 70°C) in the absence and presence of 1 mM FMN | Sulfurisphaera tokodaii |