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Literature summary extracted from

  • Fu, L.; Li, X.; Xiao, X.; Xu, J.
    Purification and characterization of a thermostable aliphatic amidase from the hyperthermophilic archaeon Pyrococcus yayanosii CH1 (2014), Extremophiles, 18, 429-440.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.4 overexpression in Escherichia coli C41 (DE3) Pyrococcus yayanosii

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.4 acetone 25% (v/v), 69% inhibition Pyrococcus yayanosii
3.5.1.4 Ca2+ 10 mM, 12% inhibition Pyrococcus yayanosii
3.5.1.4 Co2+ 10 mM, 41% inhibition Pyrococcus yayanosii
3.5.1.4 Cu2+ 10 mM, 69% inhibition Pyrococcus yayanosii
3.5.1.4 ethanol 25% (v/v), 45% inhibition Pyrococcus yayanosii
3.5.1.4 Fe3+ 10 mM, 68% inhibition Pyrococcus yayanosii
3.5.1.4 Isopropanol 25% (v/v), 54% inhibition Pyrococcus yayanosii
3.5.1.4 methanol 25% (v/v), 33% inhibition Pyrococcus yayanosii
3.5.1.4 Ni2+ 10 mM, 31% inhibition Pyrococcus yayanosii
3.5.1.4 Zn2+ 10 mM, 62% inhibition Pyrococcus yayanosii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.4 8.33
-
 acetamide pH 7.6, 60°C Pyrococcus yayanosii
3.5.1.4 10.86
-
 Acrylamide pH 7.6, 60°C Pyrococcus yayanosii
3.5.1.4 11.08
-
 hexanamide pH 7.6, 60°C Pyrococcus yayanosii
3.5.1.4 15.92
-
 L-glutamine pH 7.6, 60°C Pyrococcus yayanosii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.4 Mg2+ 10 mM, 1.15fold stimulation Pyrococcus yayanosii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.4 34000
-
 2 * 34000, SDS-PAGE Pyrococcus yayanosii
3.5.1.4 70000
-
 gel filtration Pyrococcus yayanosii

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Pyrococcus yayanosii F8AEP0
-
-
3.5.1.4 Pyrococcus yayanosii CH1 F8AEP0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.4
-
 Pyrococcus yayanosii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.4 67
-
 acrylamide: pH 7.6, 60°C Pyrococcus yayanosii
3.5.1.4 143
-
 hexanamide: pH 7.6, 60°C Pyrococcus yayanosii
3.5.1.4 1000
-
 substrate: acetamide, pH 7.6, 60°C Pyrococcus yayanosii
3.5.1.4 1430
-
 L-glutamine: pH 7.6, 60°C Pyrococcus yayanosii

Storage Stability

EC Number Storage Stability Organism
3.5.1.4 4°C, the purified enzyme can be stored for longer than 6 months at in 50 mM phosphate buffer and 10% glycerol without any loss of activity Pyrococcus yayanosii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 acetamide + H2O
-
 Pyrococcus yayanosii acetate + NH3
-
 ?
3.5.1.4 acrylamide + H2O
-
 Pyrococcus yayanosii acrylic acid + NH3
-
 ?
3.5.1.4 acrylamide + H2O
-
 Pyrococcus yayanosii CH1 acrylic acid + NH3
-
 ?
3.5.1.4 formamide + H2O 22.5% of the activity with acetamide Pyrococcus yayanosii formate + NH3
-
 ?
3.5.1.4 formamide + H2O 22.5% of the activity with acetamide Pyrococcus yayanosii CH1 formate + NH3
-
 ?
3.5.1.4 hexanamide + H2O 38.7% of the activity with acetamide Pyrococcus yayanosii hexanoate + NH3
-
 ?
3.5.1.4 L-asparagine + H2O 4.8% of the activity with acetamide Pyrococcus yayanosii L-aspartate + NH3
-
 ?
3.5.1.4 L-asparagine + H2O 4.8% of the activity with acetamide Pyrococcus yayanosii CH1 L-aspartate + NH3
-
 ?
3.5.1.4 L-glutamine + H2O 73% of the activity with acetamide Pyrococcus yayanosii L-glutamate + NH3
-
 ?
3.5.1.4 L-glutamine + H2O 73% of the activity with acetamide Pyrococcus yayanosii CH1 L-glutamate + NH3
-
 ?
3.5.1.4 additional information no activity with: urea, benzamide, pyrazinamide, nicotinamide, cinnamamide, salicylamide Pyrococcus yayanosii ?
-
 ?
3.5.1.4 additional information no activity with: urea, benzamide, pyrazinamide, nicotinamide, cinnamamide, salicylamide Pyrococcus yayanosii CH1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.4 dimer 2 * 34000, SDS-PAGE Pyrococcus yayanosii

Synonyms

EC Number Synonyms Comment Organism
3.5.1.4 AmiE
-
 Pyrococcus yayanosii
3.5.1.4 PYCH_10400 locus name Pyrococcus yayanosii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.5.1.4 85
-
 with acetamide as the substrate Pyrococcus yayanosii

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.5.1.4 50 95 50°C: 53% of maximal activity, 60°C: about 80% of maximal activity, 70°C: about 90% of maximal activity, 95°C: about 89% of maximal activity Pyrococcus yayanosii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.5.1.4 80
-
 t1/2: 110 min Pyrococcus yayanosii
3.5.1.4 90
-
 t1/2: 40 min Pyrococcus yayanosii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.1.4 333.3
-
 Acrylamide pH 7.6, 60°C Pyrococcus yayanosii
3.5.1.4 721.7
-
 hexanamide pH 7.6, 60°C Pyrococcus yayanosii
3.5.1.4 5051
-
 acetamide pH 7.6, 60°C Pyrococcus yayanosii
3.5.1.4 7216
-
 L-glutamine pH 7.6, 60°C Pyrococcus yayanosii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.4 6
-
 with acetamide as the substrate Pyrococcus yayanosii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.4 3 8 pH 3.0-5.0: about 50% of maximal activity, pH 8.0: about 65% of maximal activity Pyrococcus yayanosii

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.5.1.4 6
-
 2 h, stable Pyrococcus yayanosii
3.5.1.4 7
-
 120 min, over 60% of maximal activity is retained Pyrococcus yayanosii
3.5.1.4 8
-
 120 min, over 60% of maximal activity is retained Pyrococcus yayanosii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.5.1.4 30.69
-
 Acrylamide pH 7.6, 60°C Pyrococcus yayanosii
3.5.1.4 65.14
-
 hexanamide pH 7.6, 60°C Pyrococcus yayanosii
3.5.1.4 453.2
-
 L-glutamine pH 7.6, 60°C Pyrococcus yayanosii
3.5.1.4 606.3
-
 acetamide pH 7.6, 60°C Pyrococcus yayanosii