EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.1 | expression of mutant enzymes: P103A, P122A, S123A, H125A, S158A, R159A, G163A, H165A, P167A, and D169A in Escherichia coli. The expression level varies from 49 to 165% of that of the wild type enzyme | Sulfurisphaera tokodaii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.6.1.1 | D169A | mutant enzyme shows 1.8% of wild-type pyrophosphatase activity | Sulfurisphaera tokodaii |
3.6.1.1 | G163A | mutant enzyme shows 3.1% of wild-type pyrophosphatase activity | Sulfurisphaera tokodaii |
3.6.1.1 | H125A | mutant enzyme shows 7.5% of wild-type pyrophosphatase activity | Sulfurisphaera tokodaii |
3.6.1.1 | H165A | mutant enzyme shows 0.4% of wild-type pyrophosphatase activity | Sulfurisphaera tokodaii |
3.6.1.1 | P103A | mutant enzyme shows 3.3% of wild-type pyrophosphatase activity | Sulfurisphaera tokodaii |
3.6.1.1 | P122A | mutant enzyme shows 8.0% of wild-type pyrophosphatase activity | Sulfurisphaera tokodaii |
3.6.1.1 | P167A | mutant enzyme shows 1.8% of wild-type pyrophosphatase activity | Sulfurisphaera tokodaii |
3.6.1.1 | R159A | mutant enzyme shows 1.1% of wild-type pyrophosphatase activity | Sulfurisphaera tokodaii |
3.6.1.1 | S123A | mutant enzyme shows 16% of wild-type pyrophosphatase activity | Sulfurisphaera tokodaii |
3.6.1.1 | S158A | mutant enzyme shows 2.0% of wild-type pyrophosphatase activity | Sulfurisphaera tokodaii |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.6.1.1 | membrane | membrane-bound enzyme | Sulfurisphaera tokodaii | 16020 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.1 | additional information | Sulfurisphaera tokodaii | the enzyme is possibly involved in glycoprotein biosynthesis | ? | - |
? | |
3.6.1.1 | additional information | Sulfurisphaera tokodaii 7 | the enzyme is possibly involved in glycoprotein biosynthesis | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.1 | Sulfurisphaera tokodaii | F9VPB8 | - |
- |
3.6.1.1 | Sulfurisphaera tokodaii 7 | F9VPB8 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.1 | diphosphate + H2O | - |
Sulfurisphaera tokodaii | 2 phosphate | - |
? | |
3.6.1.1 | diphosphate + H2O | - |
Sulfurisphaera tokodaii 7 | 2 phosphate | - |
? | |
3.6.1.1 | additional information | the enzyme is possibly involved in glycoprotein biosynthesis | Sulfurisphaera tokodaii | ? | - |
? | |
3.6.1.1 | additional information | the enzyme is possibly involved in glycoprotein biosynthesis | Sulfurisphaera tokodaii 7 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.6.1.1 | ST2226 | locus name | Sulfurisphaera tokodaii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.6.1.1 | 70 | - |
assay at | Sulfurisphaera tokodaii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.6.1.1 | 3 | - |
assay at | Sulfurisphaera tokodaii |