EC Number | Application | Comment | Organism |
---|---|---|---|
1.8.1.B1 | medicine | the enzyme is a promising target for anti Fasciola treatments | Fasciola hepatica |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.1.B1 | expressed in Escherichia coli BL21 (DE3) cells | Fasciola hepatica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.1.B1 | 66000 | - |
x * 66000, SDS-PAGE | Fasciola hepatica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.B1 | GSSG + NADPH + H+ | Fasciola hepatica | - |
GSH + NADP+ | - |
? | |
1.8.1.B1 | thioredoxin disulfide + NADPH + H+ | Fasciola hepatica | - |
thioredoxin + NADP+ | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.B1 | Fasciola hepatica | A8E0R8 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.1.B1 | ammonium sulfate precipitation, Ni-NTA column chromatography, GSH-Sepharose column chromatography, and 2',5'-ADP-Sepharose column chromatography | Fasciola hepatica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.B1 | 5,5'-dithiobis(2-nitrobenzoic acid) + NADPH + H+ | - |
Fasciola hepatica | 2-nitro-5-thiobenzoate + NADP+ | - |
? | |
1.8.1.B1 | GSSG + NADPH + H+ | - |
Fasciola hepatica | GSH + NADP+ | - |
? | |
1.8.1.B1 | thioredoxin disulfide + NADPH + H+ | - |
Fasciola hepatica | thioredoxin + NADP+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.1.B1 | ? | x * 66000, SDS-PAGE | Fasciola hepatica |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.1.B1 | TGR | - |
Fasciola hepatica |
1.8.1.B1 | thioredoxin-glutathione reductase | - |
Fasciola hepatica |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.B1 | FAD | the FAD content of the recombinant enzyme is approximately 25% | Fasciola hepatica | |
1.8.1.B1 | NADPH | - |
Fasciola hepatica |