EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.368 | overexpressed in Escherichia coli | Thauera aromatica |
3.7.1.21 | overexpressed in Escherichia coli | Thauera aromatica |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.368 | additional information | the enzymew is insensitive to 10 mM EDTA, 1 mM 2,2'-bipyridyl, or 1 mM pyrazole | Thauera aromatica |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.368 | 0.06 | - |
6-hydroxycyclohex-1-ene-1-carbonyl-CoA | pH 7.4, 37°C | Thauera aromatica |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.368 | additional information | activity is not increased after adding 2-20 mM of ZnCl2 | Thauera aromatica |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.1.1.368 | 38000 | - |
x * 38000, SDS-PAGE | Thauera aromatica |
1.1.1.368 | 78000 | - |
gel filtration | Thauera aromatica |
3.7.1.21 | 40000 | - |
4 * 40000, SDS-PAGE | Thauera aromatica |
3.7.1.21 | 170000 | - |
- |
Thauera aromatica |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.368 | 6-hydroxycyclohex-1-ene-1-carbonyl-CoA + NAD+ | Thauera aromatica | the enzyme participates in the central benzoyl-CoA degradation pathway of some anaerobic bacteria | 6-oxocyclohex-1-ene-1-carbonyl-CoA + NADH + H+ | - |
ir | |
3.7.1.21 | 6-oxocyclohex-1-ene-1-carbonyl-CoA + 2 H2O | Thauera aromatica | the enzyme participates in the anaerobic benzoyl-CoA degradation pathway | 3-hydroxypimeloyl-CoA | 3-hydroxypimeloyl-CoA i.e. 3-hydroxy-6-carboxyhexanoyl-CoA | ? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.368 | Thauera aromatica | O87871 | - |
- |
3.7.1.21 | Thauera aromatica | O87872 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.1.1.368 | recombinant protein | Thauera aromatica |
3.7.1.21 | - |
Thauera aromatica |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.7.1.21 | 2-hydroxy-6-oxocyclohexane-1-carbonyl-CoA + H2O = 3-hydroxypimeloyl-CoA | (1b) | Thauera aromatica | |
3.7.1.21 | 6-oxocyclohex-1-ene-1-carbonyl-CoA + 2 H2O = 3-hydroxypimeloyl-CoA | (overall reaction) | Thauera aromatica | |
3.7.1.21 | 6-oxocyclohex-1-ene-1-carbonyl-CoA + H2O = 2-hydroxy-6-oxocyclohexane-1-carbonyl-CoA | (1a) | Thauera aromatica |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.1.1.368 | culture condition | when cells are grown aerobically with benzoate the activity is 20-25fold lower than in cells grown anaerobically with benzoate | Thauera aromatica | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.1.1.368 | 11.8 | - |
pH 7.4, 37°C, substrate: 6-hydroxycyclohex-1-ene-1-carbonyl-CoA | Thauera aromatica |
3.7.1.21 | 1.3 | - |
pH 7.4, 37°C, substrate: 6-oxocyclohex-1-ene-1-carbonyl-CoA | Thauera aromatica |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.368 | 6-hydroxycyclohex-1-ene-1-carbonyl-CoA + NAD+ | the enzyme participates in the central benzoyl-CoA degradation pathway of some anaerobic bacteria | Thauera aromatica | 6-oxocyclohex-1-ene-1-carbonyl-CoA + NADH + H+ | - |
ir | |
1.1.1.368 | 6-hydroxycyclohex-1-ene-1-carbonyl-CoA + NAD+ | the enzyme is specific for NAD+, no activity with NADP+. The enzyme is specific for 6-hydroxycyclohex-1-ene-1-carbonyl-CoA, no activity with 2-hydroxycyclohexane-1-carbonyl-CoA | Thauera aromatica | 6-oxocyclohex-1-ene-1-carbonyl-CoA + NADH + H+ | - |
ir | |
3.7.1.21 | 6-oxocyclohex-1-ene-1-carbonyl-CoA + 2 H2O | the enzyme participates in the anaerobic benzoyl-CoA degradation pathway | Thauera aromatica | 3-hydroxypimeloyl-CoA | 3-hydroxypimeloyl-CoA i.e. 3-hydroxy-6-carboxyhexanoyl-CoA | ? | |
3.7.1.21 | 6-oxocyclohex-1-ene-1-carbonyl-CoA + 2 H2O | the enzyme catalyses the addition of one molecule of water to the double bound of 6-oxocyclohex-1-ene-1-carbonyl-CoA followed by the hydrolytic C-C cleavage of the alicyclic ring | Thauera aromatica | 3-hydroxypimeloyl-CoA | 3-hydroxypimeloyl-CoA i.e. 3-hydroxy-6-carboxyhexanoyl-CoA | ? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.1.1.368 | ? | x * 38000, SDS-PAGE | Thauera aromatica |
3.7.1.21 | tetramer | 4 * 40000, SDS-PAGE | Thauera aromatica |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.368 | HAD | - |
Thauera aromatica |
3.7.1.21 | 6-oxocyclohex-1-ene-1-carbonyl-CoA hydrolase | - |
Thauera aromatica |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.368 | 37 | - |
assay at | Thauera aromatica |
3.7.1.21 | 37 | - |
assay at | Thauera aromatica |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.1.1.368 | 7 | - |
6-hydroxycyclohex-1-ene-1-carbonyl-CoA | pH 7.4, 37°C, calculated as kcat number per subunit | Thauera aromatica | |
3.7.1.21 | additional information | - |
6-oxocyclohex-1-ene-1-carbonyl-CoA | 0.9/s per subunit | Thauera aromatica |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.368 | 7.2 | 7.4 | - |
Thauera aromatica |
1.1.1.368 | 7.4 | - |
assay at | Thauera aromatica |
3.7.1.21 | 7.4 | - |
assay at | Thauera aromatica |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.368 | NAD+ | the enzyme is specific for NAD+, no activity with NADP+ | Thauera aromatica |
EC Number | Organism | Comment | Expression |
---|---|---|---|
1.1.1.368 | Thauera aromatica | expression of the enzyme activity is under negative control by oxygen | down |
1.1.1.368 | Thauera aromatica | gene expression is positively controlled by benzoate, an intermediate of the central benzoyl-CoA pathway | up |
EC Number | General Information | Comment | Organism |
---|---|---|---|
3.7.1.21 | physiological function | the enzyme participates in the anaerobic benzoyl-CoA degradation pathway | Thauera aromatica |