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Literature summary extracted from

  • Bismuto, E.; Nucci, R.; Febbraio, F.; Tanfani, F.; Gentile, F.; Briante, R.; Scire, A.; Bertoli, E.; Amodeo, P.
    Effects induced by mono- and divalent cations on protein regions responsible for thermal adaptation in beta-glycosidase from Sulfolobus solfataricus (2004), Eur. Biophys. J., 33, 38-49.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.B26 Ba2+ the inhibition exerted by the cations varies according to the following order: K+, Na+, Li+, Ba2+, Ca2+, Mg2+ Saccharolobus solfataricus
3.2.1.B26 Ca2+ the inhibition exerted by the cations varies according to the following order: K+, Na+, Li+, Ba2+, Ca2+, Mg2+ Saccharolobus solfataricus
3.2.1.B26 K+ the inhibition exerted by the cations varies according to the following order: K+, Na+, Li+, Ba2+, Ca2+, Mg2+ Saccharolobus solfataricus
3.2.1.B26 Li+ the inhibition exerted by the cations varies according to the following order: K+, Na+, Li+, Ba2+, Ca2+, Mg2+ Saccharolobus solfataricus
3.2.1.B26 Mg2+ the inhibition exerted by the cations varies according to the following order: K+, Na+, Li+, Ba2+, Ca2+, Mg2+ Saccharolobus solfataricus
3.2.1.B26 additional information cations can cause a strong attenuation of the ion pair interactions E474–K72 and D473–R402, with consequent partial dissociation of the tetrameric structure Saccharolobus solfataricus
3.2.1.B26 Na+ the inhibition exerted by the cations varies according to the following order: K+, Na+, Li+, Ba2+, Ca2+, Mg2+ Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.B26 0.84
-
 4-nitrophenyl beta-D-galactopyranoside pH 7.5, 75°C Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B26 Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B26 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + beta-D-galactopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.B26 tetramer
-
 Saccharolobus solfataricus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.B26 Sbetagly
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B26 75
-
 assay at Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B26 additional information
-
 the thermostability is affected by the nature and charge of the cations, reaching maximal effects for the case of Mg2+. Cations can cause a strong attenuation of the ion pair interactions E474–K72 and D473–R402, with consequent partial dissociation of the tetrameric structure Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.B26 445.6
-
 4-nitrophenyl beta-D-galactopyranoside pH 7.5, 75°C Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B26 7.5
-
 assay at Saccharolobus solfataricus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.B26 additional information
-
 K+ above 200 mM, pH 7.5, 75°C Saccharolobus solfataricus
3.2.1.B26 12 13 Mg2+ pH 7.5, 75°C Saccharolobus solfataricus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.B26 530.4
-
 4-nitrophenyl beta-D-galactopyranoside pH 7.5, 75°C Saccharolobus solfataricus