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Literature summary extracted from
- Identification of substrate-binding and selectivity-related residues of maltooligosyltrehalose synthase from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092 (2014), Enzyme Microb. Technol., 56, 53-59.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.15 | wild-type and mutant enzymes are expressed in Escherichia coli Rosetta (DE3) | Saccharolobus solfataricus |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.99.15 | A406 | compared with wild-type enzyme, the hydrolysis:transglycosylation selectivity ratio is significantly increased | Saccharolobus solfataricus |
| 5.4.99.15 | A406S | the hydrolysis/transglycosylation selectivity ratio shows little change | Saccharolobus solfataricus |
| 5.4.99.15 | D411A | significant reductions in catalytic efficiency, increase in the transition-state energy | Saccharolobus solfataricus |
| 5.4.99.15 | D411A | significant reductions in catalytic efficiency and increase in the transition-state energy | Saccharolobus solfataricus |
| 5.4.99.15 | D610A | significant reductions in catalytic efficiency, increase in the transition-state energy | Saccharolobus solfataricus |
| 5.4.99.15 | D610A | significant reductions in catalytic efficiency and increase in the transition-state energy | Saccharolobus solfataricus |
| 5.4.99.15 | P402 | compared with wild-type enzyme, the hydrolysis:transglycosylation selectivity ratio is significantly decreased | Saccharolobus solfataricus |
| 5.4.99.15 | P402Q | the hydrolysis/transglycosylation selectivity ratio is significantly decreased | Saccharolobus solfataricus |
| 5.4.99.15 | R614A | significant reductions in catalytic efficiency, increase in the transition-state energy | Saccharolobus solfataricus |
| 5.4.99.15 | R614A | significant reductions in catalytic efficiency and increase in the transition-state energy | Saccharolobus solfataricus |
| 5.4.99.15 | V426 | little change in hydrolysis:transglycosylation selectivity ratio | Saccharolobus solfataricus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.15 | 6.6 | - |
maltopentaose | pH 5, 60°C, wild-type enzyme | Saccharolobus solfataricus |  |
| 5.4.99.15 | 6.6 | - |
maltopentaose | pH 5.0, 60°C, wild-type enzyme | Saccharolobus solfataricus | |
| 5.4.99.15 | 9.74 | - |
maltopentaose | pH 5.0, 60°C, mutant enzyme P402Q | Saccharolobus solfataricus | |
| 5.4.99.15 | 10.1 | - |
maltopentaose | pH 5.0, 60°C, mutant enzyme A406S | Saccharolobus solfataricus | |
| 5.4.99.15 | 12.6 | - |
maltopentaose | pH 5.0, 60°C, mutant enzyme V426T | Saccharolobus solfataricus | |
| 5.4.99.15 | 43.2 | - |
maltopentaose | pH 5, 60°C, mutant enzyme D411A | Saccharolobus solfataricus | |
| 5.4.99.15 | 43.2 | - |
maltopentaose | pH 5.0, 60°C, mutant enzyme D411A | Saccharolobus solfataricus | |
| 5.4.99.15 | 43.5 | - |
maltopentaose | pH 5, 60°C, mutant enzyme R614A | Saccharolobus solfataricus | |
| 5.4.99.15 | 43.5 | - |
maltopentaose | pH 5.0, 60°C, mutant enzyme R614A | Saccharolobus solfataricus | |
| 5.4.99.15 | 122 | - |
maltopentaose | pH 5, 60°C, mutant enzyme D610A | Saccharolobus solfataricus | |
| 5.4.99.15 | 122 | - |
maltopentaose | pH 5.0, 60°C, mutant enzyme D610A | Saccharolobus solfataricus | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.15 | additional information | Saccharolobus solfataricus | key enzyme in the synthesis of trehalose | ? | - |
? | |
| 5.4.99.15 | additional information | Saccharolobus solfataricus P2 | key enzyme in the synthesis of trehalose | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.15 | Saccharolobus solfataricus | - |
- |
- |
| 5.4.99.15 | Saccharolobus solfataricus | Q7LX98 | - |
- |
| 5.4.99.15 | Saccharolobus solfataricus ATCC 35092 | - |
- |
- |
| 5.4.99.15 | Saccharolobus solfataricus P2 | Q7LX98 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.15 | Maltopentaose | the enzyme also cytalyzes the hydrolysis of maltopentaose | Saccharolobus solfataricus | alpha-Maltotriosyl trehalose | i.e. alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->1)-alpha-D-Glcp | ? | |
| 5.4.99.15 | Maltopentaose | the enzyme also cytalyzes the hydrolysis of maltopentaose | Saccharolobus solfataricus P2 | alpha-Maltotriosyl trehalose | i.e. alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->4)-alpha-D-Glcp-(1->1)-alpha-D-Glcp | ? | |
| 5.4.99.15 | maltopentaose | - |
Saccharolobus solfataricus | ? | - |
? | |
| 5.4.99.15 | maltopentaose | - |
Saccharolobus solfataricus ATCC 35092 | ? | - |
? | |
| 5.4.99.15 | additional information | key enzyme in the synthesis of trehalose | Saccharolobus solfataricus | ? | - |
? | |
| 5.4.99.15 | additional information | key enzyme in the synthesis of trehalose | Saccharolobus solfataricus P2 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.15 | maltooligosyltrehalose synthase | - |
Saccharolobus solfataricus |
| 5.4.99.15 | MTSase | - |
Saccharolobus solfataricus |
| 5.4.99.15 | SSO2095 | locus name | Saccharolobus solfataricus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.15 | 60 | - |
assay at | Saccharolobus solfataricus |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.15 | 13.4 | - |
maltopentaose | pH 5, 60°C, mutant enzyme R614A | Saccharolobus solfataricus | |
| 5.4.99.15 | 13.4 | - |
maltopentaose | pH 5.0, 60°C, mutant enzyme R614A | Saccharolobus solfataricus | |
| 5.4.99.15 | 30.2 | - |
maltopentaose | pH 5, 60°C, mutant enzyme D411A | Saccharolobus solfataricus | |
| 5.4.99.15 | 30.2 | - |
maltopentaose | pH 5.0, 60°C, mutant enzyme D411A | Saccharolobus solfataricus | |
| 5.4.99.15 | 87.2 | - |
maltopentaose | pH 5, 60°C, mutant enzyme D610A | Saccharolobus solfataricus | |
| 5.4.99.15 | 87.2 | - |
maltopentaose | pH 5.0, 60°C, mutant enzyme D610A | Saccharolobus solfataricus | |
| 5.4.99.15 | 280 | - |
maltopentaose | pH 5.0, 60°C, mutant enzyme P402Q | Saccharolobus solfataricus | |
| 5.4.99.15 | 357 | - |
maltopentaose | pH 5.0, 60°C, mutant enzyme V426T | Saccharolobus solfataricus | |
| 5.4.99.15 | 411 | - |
maltopentaose | pH 5, 60°C, wild-type enzyme | Saccharolobus solfataricus | |
| 5.4.99.15 | 411 | - |
maltopentaose | pH 5.0, 60°C, wild-type enzyme | Saccharolobus solfataricus | |
| 5.4.99.15 | 530 | - |
maltopentaose | pH 5.0, 60°C, mutant enzyme A406S | Saccharolobus solfataricus | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.15 | 5 | - |
assay at | Saccharolobus solfataricus |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 5.4.99.15 | physiological function | key enzyme in the synthesis of trehalose | Saccharolobus solfataricus |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.15 | 0.31 | - |
maltopentaose | pH 5, 60°C, mutant enzyme R614A | Saccharolobus solfataricus | |
| 5.4.99.15 | 0.31 | - |
maltopentaose | pH 5.0, 60°C, mutant enzyme R614A | Saccharolobus solfataricus | |
| 5.4.99.15 | 0.7 | - |
maltopentaose | pH 5, 60°C, mutant enzyme D411A | Saccharolobus solfataricus | |
| 5.4.99.15 | 0.7 | - |
maltopentaose | pH 5.0, 60°C, mutant enzyme D411A | Saccharolobus solfataricus | |
| 5.4.99.15 | 0.71 | - |
maltopentaose | pH 5, 60°C, mutant enzyme D610A | Saccharolobus solfataricus | |
| 5.4.99.15 | 0.71 | - |
maltopentaose | pH 5.0, 60°C, mutant enzyme D610A | Saccharolobus solfataricus | |
| 5.4.99.15 | 28.3 | - |
maltopentaose | pH 5.0, 60°C, mutant enzyme V426T | Saccharolobus solfataricus | |
| 5.4.99.15 | 28.8 | - |
maltopentaose | pH 5.0, 60°C, mutant enzyme P402Q | Saccharolobus solfataricus | |
| 5.4.99.15 | 52.5 | - |
maltopentaose | pH 5.0, 60°C, mutant enzyme A406S | Saccharolobus solfataricus | |
| 5.4.99.15 | 62.3 | - |
maltopentaose | pH 5, 60°C, wild-type enzyme | Saccharolobus solfataricus | |
| 5.4.99.15 | 62.3 | - |
maltopentaose | pH 5.0, 60°C, wild-type enzyme | Saccharolobus solfataricus | |
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