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Literature summary extracted from

  • Pozdnyakova, N.; Makarov, O.; Chernyshova, M.; Turkovskaya, O.; Jarosz-Wilkolazka, A.
    Versatile peroxidase of Bjerkandera fumosa: substrate and inhibitor specificity (2013), Enzyme Microb. Technol., 52, 44-53.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.16 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) substrate inhibition Bjerkandera fumosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.16 0.009
-
2,7-diaminofluorene absence of Mn2+, pH 4.5, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 0.035
-
syringaldazine presence of Mn2+, pH 7.0, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 0.038
-
2,7-diaminofluorene presence of Mn2+, pH not specified in the publication, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 0.063
-
2,6-dimethoxyphenol presence of Mn2+, pH 7.0, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 0.066
-
syringaldazine absence of Mn2+, pH 7.0, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 0.304
-
veratryl alcohol absence of Mn2+, pH 3.0, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 0.345
-
2,6-dimethoxyphenol absence of Mn2+, pH not specified in the publication, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 0.48
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) absence of Mn2+, pH 3.0, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 0.53
-
veratryl alcohol presence of Mn2+, pH 4.5, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 0.68
-
Mn2+ pH not specified in the publication, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 0.86
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) presence of Mn2+, pH 3.0, temperature not specified in the publication Bjerkandera fumosa

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.16 Bjerkandera fumosa
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.16 2 2,6-dimethoxyphenol + 2 H2O2
-
Bjerkandera fumosa coerulignone + 2 H2O
-
?
1.11.1.16 2 Mn2+ + 2 H+
-
Bjerkandera fumosa 2 Mn3+ + H2
-
?
1.11.1.16 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
Bjerkandera fumosa ? + H2O
-
?
1.11.1.16 2,7-diaminofluorene + H2O2 during oxidation of 2,7-diaminofluorene, both with and without Mn2+, biphasic kinetics with apparent saturation in both micromolar and millimolar ranges are obtained Bjerkandera fumosa ? + H2O
-
?
1.11.1.16 4-aminobenzoic acid + H2O2
-
Bjerkandera fumosa ? + H2O
-
?
1.11.1.16 anthracene + H2O2
-
Bjerkandera fumosa 9,10-anthraquinone + H2O
-
?
1.11.1.16 chrysene + H2O2
-
Bjerkandera fumosa ? + H2O
-
?
1.11.1.16 fluoranthene + H2O2
-
Bjerkandera fumosa ? + H2O
-
?
1.11.1.16 fluorene + H2O2
-
Bjerkandera fumosa 9-fluorenone + H2O
-
?
1.11.1.16 additional information oxidation of substrates may occur in two ways, either directly by the enzyme or by diffusible chelated Mn3+ as an oxidizing agent Bjerkandera fumosa ?
-
?
1.11.1.16 phenanthrene + H2O2
-
Bjerkandera fumosa 9,10-phenanthrenequinone + H2O
-
?
1.11.1.16 pyrene + H2O2
-
Bjerkandera fumosa ? + H2O
-
?
1.11.1.16 syringaldazine + H2O2
-
Bjerkandera fumosa ?
-
?
1.11.1.16 veratryl alcohol + H2O2 + H+
-
Bjerkandera fumosa veratraldehyde + H2O
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.16 0.19
-
2,7-diaminofluorene absence of Mn2+, pH 4.5, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 0.22
-
syringaldazine absence of Mn2+, pH 7.0, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 0.83
-
2,7-diaminofluorene presence of Mn2+, pH 7.0, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 2.77
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) absence of Mn2+, pH 3.0, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 3.9
-
Mn2+ pH not specified in the publication, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 6.1
-
syringaldazine presence of Mn2+, pH 7.0, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 6.2
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) presence of Mn2+, pH 3.0, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 7.08
-
veratryl alcohol absence of Mn2+, pH 3.0, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 10.6
-
2,6-dimethoxyphenol absence of Mn2+, pH not specified in the publication, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 26.8
-
veratryl alcohol presence of Mn2+, pH 4.5, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 30
-
2,6-dimethoxyphenol presence of Mn2+, pH not specified in the publication, temperature not specified in the publication Bjerkandera fumosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.16 3
-
and 4.2, substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), presence of Mn2+ Bjerkandera fumosa
1.11.1.16 3
-
and 6.5, substrate veratryl alcohol, absence of Mn2+ Bjerkandera fumosa
1.11.1.16 3
-
substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), absence of Mn2+ Bjerkandera fumosa
1.11.1.16 4.2
-
and 3.0, substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), presence of Mn2+ Bjerkandera fumosa
1.11.1.16 4.5
-
and 7.0, substrate 2,7-diaminofluorene, absence of Mn2+ Bjerkandera fumosa
1.11.1.16 4.5
-
substrate veratryl alcohol, presence of Mn2+ Bjerkandera fumosa
1.11.1.16 5
-
and 6.5, substrate 2,7-diaminofluorene, presence of Mn2+ Bjerkandera fumosa
1.11.1.16 6.5
-
and 3.0, substrate veratryl alcohol, absence of Mn2+ Bjerkandera fumosa
1.11.1.16 6.5
-
and 3.0, substrate veratryl alcohol, presence of Mn2+ Bjerkandera fumosa
1.11.1.16 6.5
-
and 5.0, substrate 2,7-diaminofluorene, presence of Mn2+ Bjerkandera fumosa
1.11.1.16 7
-
and 4.5, substrate 2,7-diaminofluorene, absence of Mn2+ Bjerkandera fumosa
1.11.1.16 7
-
substrate syringaldazine, absence and presence of Mn2+ Bjerkandera fumosa
1.11.1.16 7
-
substrate syringaldazine, absence of Mn2+ Bjerkandera fumosa

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.16 2.3
-
veratryl alcohol absence of Mn2+, pH 3.0, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 3.3
-
syringaldazine absence of Mn2+, pH 7.0, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 5.7
-
Mn2+ pH not specified in the publication, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 5.8
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) absence of Mn2+, pH 3.0, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 7.2
-
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) presence of Mn2+, pH 3.0, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 21.1
-
2,7-diaminofluorene absence of Mn2+, pH 4.5, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 21.8
-
2,7-diaminofluorene presence of Mn2+, pH 7.0, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 30.7
-
2,6-dimethoxyphenol absence of Mn2+, pH not specified in the publication, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 50.6
-
veratryl alcohol presence of Mn2+, pH 4.5, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 174.3
-
syringaldazine presence of Mn2+, pH 7.0, temperature not specified in the publication Bjerkandera fumosa
1.11.1.16 476.2
-
2,6-dimethoxyphenol presence of Mn2+, pH not specified in the publication, temperature not specified in the publication Bjerkandera fumosa