Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Moeller, K.; Nguyen, G.S.; Hollmann, F.; Hanefeld, U.
    Expression and characterization of the nitrile reductase queF from E. coli (2013), Enzyme Microb. Technol., 52, 129-133.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.13 0.0002
-
 NADPH or below, pH 7.0, 37°C Escherichia coli
1.7.1.13 0.0015
-
 7-cyano-7-deazaguanine or below, pH 7.0, 37°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.13 Escherichia coli Q46920
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.13 7-cyano-7-deazaguanine + NADPH + H+
-
 Escherichia coli queuine + NADP+ + H+
-
 ?
1.7.1.13 additional information no substrates: acetonitrile, benzonitrile and benzylcyanide Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.1.13 QueF
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.13 37
-
-
 Escherichia coli

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.7.1.13 37
-
 half-life 28.2 Escherichia coli
1.7.1.13 40
-
 half-life 12.8 Escherichia coli
1.7.1.13 45
-
 half-life 2.8 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.1.13 0.0033
-
 NADPH pH 7.0, 37°C Escherichia coli
1.7.1.13 0.127
-
 7-cyano-7-deazaguanine pH 7.0, 37°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.13 7
-
 Tris-buffer Escherichia coli

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.7.1.13 7
-
 4 h, no loss of activity Escherichia coli
1.7.1.13 9
-
 4 h, 80% residual activity Escherichia coli