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Literature summary extracted from

  • Gao, X.; Ren, J.; Wu, Q.; Zhu, D.
    Biochemical characterization and substrate profiling of a new NADH-dependent enoate reductase from Lactobacillus casei (2012), Enzyme Microb. Technol., 51, 26-34.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.31 expressed in Escherichia coli Lacticaseibacillus casei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.1.31 0.034
-
 NADH pH 7.0, 30°C Lacticaseibacillus casei
1.3.1.31 1.94
-
 2-cyclohexen-1-one pH 7.0, 30°C Lacticaseibacillus casei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.31 42300
-
 calculated from cDNA Lacticaseibacillus casei
1.3.1.31 46800
-
 SDS-PAGE Lacticaseibacillus casei
1.3.1.31 170000
-
 gel filtration Lacticaseibacillus casei

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.31 Lacticaseibacillus casei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.31 using ammonium sulfate precipitation and treatment in an acidic buffer Lacticaseibacillus casei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.31 (2E,6Z)-nona-2,6-dienal + NADH + H+ relative activity: 62.7% Lacticaseibacillus casei ?
-
 ?
1.3.1.31 (E)-2-decenal + NADH + H+ relative activity: 90% Lacticaseibacillus casei decanal + NAD+
-
 ?
1.3.1.31 (E)-2-hexen-1-al + NADH + H+ relative activity: 100% Lacticaseibacillus casei hexanal + NAD+
-
 ?
1.3.1.31 (E)-2-methyl-2-butenal + NADH + H+ relative activity: 59.2% Lacticaseibacillus casei 2-methylbutanal + NAD+
-
 ?
1.3.1.31 (E)-2-nonenal + NADH + H+ relative activity: 57% Lacticaseibacillus casei nonanal + NAD+
-
 ?
1.3.1.31 (E)-2-octenal + NADH + H+ relative activity: 75.4% Lacticaseibacillus casei octanal + NAD+
-
 ?
1.3.1.31 (E)-2-pentenal + NADH + H+ relative activity: 45% Lacticaseibacillus casei 2-pentanone + NAD+
-
 ?
1.3.1.31 (E)-3-nonen-2-one + NADH + H+ relative activity: 36.6% Lacticaseibacillus casei nonan-2-one + NAD+
-
 ?
1.3.1.31 (R)-(-)-carvone + NADH + H+ relative activity: 70% Lacticaseibacillus casei ?
-
 ?
1.3.1.31 2-cyclohexen-1-one + NADH + H+ relative activity: 830% Lacticaseibacillus casei cyclohexanone + NAD+
-
 ?
1.3.1.31 2-cyclohexen-1-one + NADH + H+
-
 Lacticaseibacillus casei 2-cyclohexanone + NAD+
-
 ?
1.3.1.31 2-isopropyl-5-methyl-2-hexenal + NADH + H+ relative activity: 15.5% Lacticaseibacillus casei 2-isopropyl-5-methylhexanal + NAD+
-
 ?
1.3.1.31 2-methyl-2-cyclopenten-1-one + NADH + H+ relative activity: 62.7% Lacticaseibacillus casei ?
-
 ?
1.3.1.31 3-methyl-2-cyclopentenone + NADH + H+ relative activity: 31% Lacticaseibacillus casei ?
-
 ?
1.3.1.31 bromomaleic anhydride + NADH + H+ relative activity: 107% Lacticaseibacillus casei ?
-
 ?
1.3.1.31 phenylmaleic anhydride + NADH + H+ relative activity: 402% Lacticaseibacillus casei ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.31 tetramer 4 * 42300, gel filtration Lacticaseibacillus casei

Synonyms

EC Number Synonyms Comment Organism
1.3.1.31 enoate reductase
-
 Lacticaseibacillus casei
1.3.1.31 LacER
-
 Lacticaseibacillus casei

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.3.1.31 50 55 temp. optimum Lacticaseibacillus casei

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.1.31 65
-
 no activity after 1 h Lacticaseibacillus casei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.1.31 3200
-
 NADH pH 7.0, 30°C Lacticaseibacillus casei
1.3.1.31 8400
-
 2-cyclohexen-1-one pH 7.0, 30°C Lacticaseibacillus casei

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.3.1.31 8 9 optimal pH range Lacticaseibacillus casei

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.31 NADH
-
 Lacticaseibacillus casei