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Literature summary extracted from

  • Boka, B.; Adanyi, N.; Szamos, J.; Virag, D.; Kiss, A.
    Putrescine biosensor based on putrescine oxidase from Kocuria rosea (2012), Enzyme Microb. Technol., 51, 258-262.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.4.3.10 food industry the immobilized enzyme may be used as a sensor for analysis of beer samples Kocuria rosea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.10 50000
-
 2 * 50000, SDS-PAGE Kocuria rosea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.10 putrescine + O2 + H2O Kocuria rosea 100% activity 4-aminobutanal + NH3 + H2O2
-
 ?
1.4.3.10 putrescine + O2 + H2O Kocuria rosea K22 100% activity 4-aminobutanal + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.10 Kocuria rosea
-
 formerly Micrococcus rubens
-
1.4.3.10 Kocuria rosea K22
-
 formerly Micrococcus rubens
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.10 three-phase partitioning and DEAE Sephadex gel filtration Kocuria rosea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.10 cadaverine + O2 + H2O 10.7% activity compared to putrescine Kocuria rosea 5-aminopentanal + NH3 + H2O2
-
 ?
1.4.3.10 cadaverine + O2 + H2O 10.7% activity compared to putrescine Kocuria rosea K22 5-aminopentanal + NH3 + H2O2
-
 ?
1.4.3.10 histamine + O2 + H2O 1.7% activity compared to putrescine Kocuria rosea 1H-imidazol-4-ylacetaldehyde + NH3 + H2O2
-
 ?
1.4.3.10 histamine + O2 + H2O 1.7% activity compared to putrescine Kocuria rosea K22 1H-imidazol-4-ylacetaldehyde + NH3 + H2O2
-
 ?
1.4.3.10 putrescine + O2 + H2O 100% activity Kocuria rosea 4-aminobutanal + NH3 + H2O2
-
 ?
1.4.3.10 putrescine + O2 + H2O 100% activity Kocuria rosea K22 4-aminobutanal + NH3 + H2O2
-
 ?
1.4.3.10 spermidine + O2 + H2O 5.0% activity compared to putrescine Kocuria rosea 4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
-
 ?
1.4.3.10 spermidine + O2 + H2O 5.0% activity compared to putrescine Kocuria rosea K22 4-aminobutyraldehyde + 1,3-diaminopropane + H2O2
-
 ?
1.4.3.10 tryptamine + O2 + H2O 5.2% activity compared to putrescine Kocuria rosea 1H-indol-3-yl-acetaldehyde + NH3 + H2O2
-
 ?
1.4.3.10 tyramine + O2 + H2O 2.0% activity compared to putrescine Kocuria rosea (4-hydroxyphenyl)acetaldehyde + NH3 + H2O2
-
 ?
1.4.3.10 tyramine + O2 + H2O 2.0% activity compared to putrescine Kocuria rosea K22 (4-hydroxyphenyl)acetaldehyde + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.10 homodimer 2 * 50000, SDS-PAGE Kocuria rosea

Synonyms

EC Number Synonyms Comment Organism
1.4.3.10 PUOX
-
 Kocuria rosea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.10 8 8.6
-
 Kocuria rosea

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.10 FAD
-
 Kocuria rosea