BRENDA - Enzyme Database

Amperometric glucose biosensor utilizing FAD-dependent glucose dehydrogenase immobilized on nanocomposite electrode

Monosik, R.; Stredansky, M.; Luspai, K.; Magdolen, P.; Sturdik, E.; Enzyme Microb. Technol. 50, 227-232 (2012)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
1.1.5.9
analysis
the enzyme can be used as O2-independent biosensor for glucoe detection
Aspergillus oryzae
1.1.5.9
diagnostics
the enzyme can be used as O2-independent biosensor for glucoe detection
Aspergillus oryzae
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.1.5.9
additional information
amperometric glucose biosensor utilizing FAD-dependent glucose dehydrogenase immobilized on nanocomposite electrode. Unlike the common glucose oxidase based biosensor, the presented biosensors is O2-independent, method and biosensorevlauation, overview. Polyphenols also do not interfere at used measuring conditions. Determination of D-glucose in beverages and wines using biosensors, HPLC and enzymatic-spectrophotometric assay, overview
Aspergillus oryzae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.5.9
3.09
-
D-glucose
pH 7.4, 37C, enzyme immobilized on amperometric electrode
Aspergillus oryzae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.5.9
D-glucose + a quinone
Aspergillus oryzae
-
D-glucono-1,5-lactone + a reduced quinol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.5.9
Aspergillus oryzae
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.5.9
D-glucose + a quinone
-
724876
Aspergillus oryzae
D-glucono-1,5-lactone + a reduced quinol
-
-
-
?
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.5.9
37
-
assay at
Aspergillus oryzae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.5.9
7.4
-
assay at
Aspergillus oryzae
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.5.9
5.5
7.5
-
Aspergillus oryzae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.1.5.9
FAD
dependent on
Aspergillus oryzae
Application (protein specific)
EC Number
Application
Commentary
Organism
1.1.5.9
analysis
the enzyme can be used as O2-independent biosensor for glucoe detection
Aspergillus oryzae
1.1.5.9
diagnostics
the enzyme can be used as O2-independent biosensor for glucoe detection
Aspergillus oryzae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.1.5.9
FAD
dependent on
Aspergillus oryzae
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.1.5.9
additional information
amperometric glucose biosensor utilizing FAD-dependent glucose dehydrogenase immobilized on nanocomposite electrode. Unlike the common glucose oxidase based biosensor, the presented biosensors is O2-independent, method and biosensorevlauation, overview. Polyphenols also do not interfere at used measuring conditions. Determination of D-glucose in beverages and wines using biosensors, HPLC and enzymatic-spectrophotometric assay, overview
Aspergillus oryzae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.1.5.9
3.09
-
D-glucose
pH 7.4, 37C, enzyme immobilized on amperometric electrode
Aspergillus oryzae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.1.5.9
D-glucose + a quinone
Aspergillus oryzae
-
D-glucono-1,5-lactone + a reduced quinol
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.5.9
D-glucose + a quinone
-
724876
Aspergillus oryzae
D-glucono-1,5-lactone + a reduced quinol
-
-
-
?
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
1.1.5.9
37
-
assay at
Aspergillus oryzae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.1.5.9
7.4
-
assay at
Aspergillus oryzae
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.1.5.9
5.5
7.5
-
Aspergillus oryzae
General Information
EC Number
General Information
Commentary
Organism
1.1.5.9
additional information
amperometric glucose biosensor utilizing FAD-dependent glucose dehydrogenase immobilized on nanocomposite electrode. Unlike the common glucose oxidase based biosensor, the presented biosensors is O2-independent, method and biosensorevlauation, overview. Polyphenols also do not interfere at used measuring conditions. Determination of D-glucose in beverages and wines using biosensors, HPLC and enzymatic-spectrophotometric assay, overview
Aspergillus oryzae
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.1.5.9
additional information
amperometric glucose biosensor utilizing FAD-dependent glucose dehydrogenase immobilized on nanocomposite electrode. Unlike the common glucose oxidase based biosensor, the presented biosensors is O2-independent, method and biosensorevlauation, overview. Polyphenols also do not interfere at used measuring conditions. Determination of D-glucose in beverages and wines using biosensors, HPLC and enzymatic-spectrophotometric assay, overview
Aspergillus oryzae