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Literature summary extracted from

  • Chauhan, A.; Pandey, G.; Sharma, N.K.; Paul, D.; Pandey, J.; Jain, R.K.
    p-Nitrophenol degradation via 4-nitrocatechol in Burkholderia sp. SJ98 and cloning of some of the lower pathway genes (2010), Environ. Sci. Technol., 44, 3435-3441.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.32 expressed in Escherichia coli Ralstonia sp.

General Stability

EC Number General Stability Organism
1.3.1.32 highly unstable and prone to precipitation at a concentration of 1 mg/ml or more in 50 mM Tris-Cl (pH 8.0) containing 50 mM NaCl at ambient temperatures. The stability of MaR can be improved by the addition of glycerol (at 30% v/v concentration) or by increasing the NaCl concentration (up to 1 M) Ralstonia sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.1.32 40000
-
 gel filtration Ralstonia sp.
1.3.1.32 40000
-
 SDS-PAGE Ralstonia sp.

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.32 Ralstonia sp. Q6PW32
-
-
1.3.1.32 Ralstonia sp. SJ98 Q6PW32
-
-

Storage Stability

EC Number Storage Stability Organism
1.3.1.32 -20°C, 30% glycerol, 4 months, enzyme retains its activity Ralstonia sp.
1.3.1.32 -70°C, 30% glycerol, 6 months, enzyme retains its activity Ralstonia sp.
1.3.1.32 4°C, 30% glycerol, 3 weeks, enzyme retains its activity Ralstonia sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.32 2-maleylacetate + NADPH
-
 Ralstonia sp. 3-oxoadipate + NADP+
-
 ?
1.3.1.32 2-maleylacetate + NADPH
-
 Ralstonia sp. SJ98 3-oxoadipate + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.32 monomer gel filtration Ralstonia sp.

Synonyms

EC Number Synonyms Comment Organism
1.3.1.32 maleylacetate reductase
-
 Ralstonia sp.
1.3.1.32 MAR
-
 Ralstonia sp.
1.3.1.32 PnpD
-
 Ralstonia sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.32 7.5
-
 assay at Ralstonia sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.1.32 NADH
-
 Ralstonia sp.