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Literature summary extracted from

  • Denkmann, K.; Grein, F.; Zigann, R.; Siemen, A.; Bergmann, J.; van Helmont, S.; Nicolai, A.; Pereira, I.A.; Dahl, C.
    Thiosulfate dehydrogenase: a widespread unusual acidophilic c-type cytochrome (2012), Environ. Microbiol., 14, 2673-2688.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.2.2 expression in Escherichia coli Allochromatium vinosum

Protein Variants

EC Number Protein Variants Comment Organism
1.8.2.2 C123G residue probably acts as sixth distal axial ligand of a heme iron, mutant is inactive Allochromatium vinosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.2.2 0.66
-
 ferricyanide S0.5 value, pH 4.0, 30°C Allochromatium vinosum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.2.2 periplasm
-
 Allochromatium vinosum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.2.2 25798
-
 25800, SDS-PAGE, x * 25798, calculated Allochromatium vinosum
1.8.2.2 25800
-
 25800, SDS-PAGE, x * 25798, calculated Allochromatium vinosum

Organism

EC Number Organism UniProt Comment Textmining
1.8.2.2 Allochromatium vinosum D3RVD4
-
-
1.8.2.2 Allochromatium vinosum ATCC 17899 D3RVD4
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.2.2 5800
-
 30°C, pH 5.0 Allochromatium vinosum
1.8.2.2 30000
-
 30°C, pH 4.0 Allochromatium vinosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.2.2 2 thiosulfate + 2 ferricyanide
-
 Allochromatium vinosum tetrathionate + 2 ferrocyanide
-
 ?
1.8.2.2 2 thiosulfate + 2 ferricyanide
-
 Allochromatium vinosum ATCC 17899 tetrathionate + 2 ferrocyanide
-
 ?

Subunits

EC Number Subunits Comment Organism
1.8.2.2 ? 25800, SDS-PAGE, x * 25798, calculated Allochromatium vinosum

Synonyms

EC Number Synonyms Comment Organism
1.8.2.2 Alvin_0091 locus name Allochromatium vinosum
1.8.2.2 TsdA
-
 Allochromatium vinosum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.8.2.2 14000
-
 thiosulfate pH 4.0, 30°C Allochromatium vinosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.2.2 4
-
-
 Allochromatium vinosum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.2.2 6.8
-
 no residual activity Allochromatium vinosum

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.2.2 heme diheme cytochrome c, residues methionine M222 or M236 and cysteine C123 are probable sixth distal axial ligands of the two heme irons Allochromatium vinosum

General Information

EC Number General Information Comment Organism
1.8.2.2 physiological function a gene deletion mutant does not produce any tetrathionate from thiosulfate during growth at pH 7.2 or 6.9 Allochromatium vinosum