Any feedback?
Literature summary extracted from
- High-level production of soluble pyrroloquinoline quinone-dependent glucose dehydrogenase in Escherichia coli (2012), Eng. Life Sci., 12, 574-582.
No PubMed abstract available
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.1.5.2 | diagnostics | the enzyme is attractive for application in glucose detection in clinic diagnosis and industrial bioprocess controls | Acinetobacter calcoaceticus |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.1.5.2 | recombinant high-level production of soluble His-tagged pyrroloquinoline quinone-dependent glucose dehydrogenase in Escherichia coli strain BL21 (DE3), 6fold improvement improvement of sPQQGDH expression in MMBL medium | Acinetobacter calcoaceticus |
| 1.1.99.35 | expressed in Escherichia coli BL21(DE3) cells | Acinetobacter calcoaceticus |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.1.5.2 | membrane | isozyme mPQQGDH | Acinetobacter calcoaceticus | 16020 | - |
| 1.1.5.2 | additional information | there are two types of PQQGDH, the membrane-bound glucose dehydrogenase and the soluble glucose dehydrogenase | Acinetobacter calcoaceticus | - |
- |
| 1.1.5.2 | periplasm | soluble isozyme sPQQGDH | Acinetobacter calcoaceticus | - |
- |
| 1.1.99.35 | soluble | - |
Acinetobacter calcoaceticus | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.5.2 | Ca2+ | three ions per enzyme subunit | Acinetobacter calcoaceticus |  |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.1.5.2 | 50000 | - |
2 * 50000, SDS-PAGE | Acinetobacter calcoaceticus |
| 1.1.99.35 | 50000 | - |
2 * 50000, SDS-PAGE | Acinetobacter calcoaceticus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.5.2 | D-glucose + ubiquinone | Acinetobacter calcoaceticus | preferred substrate | D-glucono-1,5-lactone + ubiquinol | - |
? | |
| 1.1.5.2 | D-glucose + ubiquinone | Acinetobacter calcoaceticus L.M.D. 79.41 | preferred substrate | D-glucono-1,5-lactone + ubiquinol | - |
? | |
| 1.1.99.35 | D-galactose + oxidized 2,6-dichlorophenolindophenol | Acinetobacter calcoaceticus | about 13% activity compared to D-glucose | D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.35 | D-galactose + oxidized 2,6-dichlorophenolindophenol | Acinetobacter calcoaceticus L.M.D. 79.41 | about 13% activity compared to D-glucose | D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.35 | D-glucose + oxidized 2,6-dichlorophenolindophenol | Acinetobacter calcoaceticus | 100% activity | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.35 | D-glucose + oxidized 2,6-dichlorophenolindophenol | Acinetobacter calcoaceticus L.M.D. 79.41 | 100% activity | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.35 | D-mannose + oxidized 2,6-dichlorophenolindophenol | Acinetobacter calcoaceticus | about 11% activity compared to D-glucose | D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.35 | D-xylose + oxidized 2,6-dichlorophenolindophenol | Acinetobacter calcoaceticus | about 9% activity compared to D-glucose | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.35 | lactose + oxidized 2,6-dichlorophenolindophenol | Acinetobacter calcoaceticus | about 50% activity compared to D-glucose | lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.35 | maltose + oxidized 2,6-dichlorophenolindophenol | Acinetobacter calcoaceticus | about 70% activity compared to D-glucose | maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.35 | additional information | Acinetobacter calcoaceticus | almost no activity with D-fructose | ? | - |
? | |
| 1.1.99.35 | additional information | Acinetobacter calcoaceticus L.M.D. 79.41 | almost no activity with D-fructose | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.1.5.2 | Acinetobacter calcoaceticus | - |
- |
- |
| 1.1.5.2 | Acinetobacter calcoaceticus L.M.D. 79.41 | - |
- |
- |
| 1.1.99.35 | Acinetobacter calcoaceticus | - |
- |
- |
| 1.1.99.35 | Acinetobacter calcoaceticus L.M.D. 79.41 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.1.5.2 | recombinant soluble His-tagged enzyme 11.3fold from Escherichia coli strain BL21 (DE3)by nickel affinity chromatography | Acinetobacter calcoaceticus |
| 1.1.99.35 | Ni-NTA affinity column chromatography | Acinetobacter calcoaceticus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 1.1.5.2 | additional information | effects of different carbon sources and glucose concentrations on the cell growth and sPQQGDH production, overview | Acinetobacter calcoaceticus | - |
| 1.1.99.35 | culture condition:D-glucose-grown cell | - |
Acinetobacter calcoaceticus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.1.5.2 | 5811 | - |
purified recombinant His-tagged enzyme, pH 7.0, 25°C | Acinetobacter calcoaceticus |
| 1.1.99.35 | 515 | - |
crude cell lysate, at pH 7.0 and 25°C | Acinetobacter calcoaceticus |
| 1.1.99.35 | 5811 | - |
after 11.3fold purification, at pH 7.0 and 25°C | Acinetobacter calcoaceticus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.1.5.2 | D-glucose + ubiquinone | preferred substrate | Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ubiquinol | - |
? | |
| 1.1.5.2 | D-glucose + ubiquinone | preferred substrate, assayed with the electron acceptors phenazine methosulfate and dichlorphenolindophenol | Acinetobacter calcoaceticus | D-glucono-1,5-lactone + ubiquinol | - |
? | |
| 1.1.5.2 | D-glucose + ubiquinone | preferred substrate | Acinetobacter calcoaceticus L.M.D. 79.41 | D-glucono-1,5-lactone + ubiquinol | - |
? | |
| 1.1.5.2 | D-glucose + ubiquinone | preferred substrate, assayed with the electron acceptors phenazine methosulfate and dichlorphenolindophenol | Acinetobacter calcoaceticus L.M.D. 79.41 | D-glucono-1,5-lactone + ubiquinol | - |
? | |
| 1.1.99.35 | D-galactose + oxidized 2,6-dichlorophenolindophenol | about 13% activity compared to D-glucose | Acinetobacter calcoaceticus | D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.35 | D-galactose + oxidized 2,6-dichlorophenolindophenol | about 13% activity compared to D-glucose | Acinetobacter calcoaceticus L.M.D. 79.41 | D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.35 | D-glucose + oxidized 2,6-dichlorophenolindophenol | 100% activity | Acinetobacter calcoaceticus | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.35 | D-glucose + oxidized 2,6-dichlorophenolindophenol | 100% activity | Acinetobacter calcoaceticus L.M.D. 79.41 | D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.35 | D-mannose + oxidized 2,6-dichlorophenolindophenol | about 11% activity compared to D-glucose | Acinetobacter calcoaceticus | D-mannono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.35 | D-xylose + oxidized 2,6-dichlorophenolindophenol | about 9% activity compared to D-glucose | Acinetobacter calcoaceticus | D-xylono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.35 | lactose + oxidized 2,6-dichlorophenolindophenol | about 50% activity compared to D-glucose | Acinetobacter calcoaceticus | lactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.35 | maltose + oxidized 2,6-dichlorophenolindophenol | about 70% activity compared to D-glucose | Acinetobacter calcoaceticus | maltono-1,5-lactone + reduced 2,6-dichlorophenolindophenol | - |
? | |
| 1.1.99.35 | additional information | almost no activity with D-fructose | Acinetobacter calcoaceticus | ? | - |
? | |
| 1.1.99.35 | additional information | almost no activity with D-fructose | Acinetobacter calcoaceticus L.M.D. 79.41 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.1.5.2 | homodimer | 2 * 50000, SDS-PAGE | Acinetobacter calcoaceticus |
| 1.1.99.35 | homodimer | 2 * 50000, SDS-PAGE | Acinetobacter calcoaceticus |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.1.5.2 | PQQGDH | - |
Acinetobacter calcoaceticus |
| 1.1.5.2 | pyrroloquinoline quinone-dependent glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
| 1.1.99.35 | PQQGDH | - |
Acinetobacter calcoaceticus |
| 1.1.99.35 | soluble pyrroloquinoline quinone-dependent glucose dehydrogenase | - |
Acinetobacter calcoaceticus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.5.2 | 25 | - |
assay at | Acinetobacter calcoaceticus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.1.5.2 | 55 | - |
purified recombinant enzyme, 10 min, stable | Acinetobacter calcoaceticus |
| 1.1.99.35 | 55 | - |
the half-life of enzyme thermal inactivation at 55°C is 12.1 min | Acinetobacter calcoaceticus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.1.5.2 | 7 | - |
assay at | Acinetobacter calcoaceticus |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.1.5.2 | pyrroloquinoline quinone | required for catalysis, one molecule per enzyme subunit | Acinetobacter calcoaceticus | |
| 1.1.99.35 | pyrroloquinoline quinone | - |
Acinetobacter calcoaceticus | |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.1.5.2 | additional information | the purified recombinant PQQGDH shows thermal stability and substrate specificity as the native enzyme | Acinetobacter calcoaceticus |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
