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Literature summary extracted from

  • Aquino Neto, S.; Suda, E.; Xu, S.; Meredith, M.; De Andrade, A.; Minteer, S.
    Direct electron transfer-based bioanodes for ethanol biofuel cells using PQQ-dependent alcohol and aldehyde dehydrogenases (2013), Electrochim. Acta, 87, 323-329.
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.5.5 biofuel production comparison of a direct electron transfer bioanode containing both PQQ-ADH (pyrroloquinoline quinone-dependent alcohol dehydrogenase) and PQQ-AldDH (PQQ-dependent aldehyde dehydrogenase) immobilized onto different modified electrode surfaces employing either a tetrabutylammonium-modified Nafion membrane polymer or polyamidoamine (PAMAM) dendrimers. The prepared bioelectrodes are able to undergo direct electron transfer onto glassy carbon surface in the presence as well as the absence of multi-walled carbon nanotubes, also, in the latter case a relevant shift in the oxidation peak of about 180 mV vs. saturated calomel electrode is observed Gluconobacter sp. 33

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.5 0.153
-
 ethanol at pH 7.2 and 25°C Gluconobacter sp.
1.1.5.5 3.86
-
 acetaldehyde at pH 7.2 and 25°C Gluconobacter sp.
1.2.5.2 3.86
-
 acetaldehyde at pH 7.2 and 25°C Gluconobacter sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.5 15000
-
-
 Gluconobacter sp.
1.1.5.5 50000
-
-
 Gluconobacter sp.
1.1.5.5 80000
-
-
 Gluconobacter sp.
1.1.5.5 145000
-
 gel filtration Gluconobacter sp.
1.2.5.2 50000
-
 1 * 80000 + 1 * 50000, SDS-PAGE Gluconobacter sp.
1.2.5.2 80000
-
 1 * 80000 + 1 * 50000, SDS-PAGE Gluconobacter sp.
1.2.5.2 141000
-
 gel filtration Gluconobacter sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.5.5 acetaldehyde + ubiquinol Gluconobacter sp.
-
 ethanol + ubiquinone
-
 r
1.1.5.5 acetaldehyde + ubiquinol Gluconobacter sp. 33 (DSM Z 3504)
-
 ethanol + ubiquinone
-
 r
1.1.5.5 acetaldehyde + ubiquinol Gluconobacter sp. DSM 3504 / ATCC 15163
-
 ethanol + ubiquinone
-
 r
1.1.5.5 ethanol + ubiquinone Gluconobacter sp.
-
 acetaldehyde + ubiquinol
-
 r
1.1.5.5 ethanol + ubiquinone Gluconobacter sp. 33 (DSM Z 3504)
-
 acetaldehyde + ubiquinol
-
 r
1.1.5.5 ethanol + ubiquinone Gluconobacter sp. DSM 3504 / ATCC 15163
-
 acetaldehyde + ubiquinol
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.5 Gluconobacter sp.
-
-
-
1.1.5.5 Gluconobacter sp. 33
-
-
-
1.1.5.5 Gluconobacter sp. DSM 3504 / ATCC 15163
-
-
-
1.2.5.2 Gluconobacter sp.
-
-
-
1.2.5.2 Gluconobacter sp. 33 (DSM Z 3504)
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.5.2 DEAE Toyo-Pearl 650M column chromatography and Sephadex G-100 gel filtration Gluconobacter sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.5.2 culture condition:D-mannitol-grown cell
-
 Gluconobacter sp.
-

Storage Stability

EC Number Storage Stability Organism
1.2.5.2 -20°C, purified enzyme, 30 days, remains stable Gluconobacter sp.
1.2.5.2 4°C, purified enzyme, few days, remains stable Gluconobacter sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.5 acetaldehyde + ubiquinol
-
 Gluconobacter sp. ethanol + ubiquinone
-
 r
1.1.5.5 acetaldehyde + ubiquinol
-
 Gluconobacter sp. 33 (DSM Z 3504) ethanol + ubiquinone
-
 r
1.1.5.5 acetaldehyde + ubiquinol
-
 Gluconobacter sp. DSM 3504 / ATCC 15163 ethanol + ubiquinone
-
 r
1.1.5.5 ethanol + ubiquinone
-
 Gluconobacter sp. acetaldehyde + ubiquinol
-
 r
1.1.5.5 ethanol + ubiquinone
-
 Gluconobacter sp. 33 (DSM Z 3504) acetaldehyde + ubiquinol
-
 r
1.1.5.5 ethanol + ubiquinone
-
 Gluconobacter sp. DSM 3504 / ATCC 15163 acetaldehyde + ubiquinol
-
 r
1.2.5.2 acetaldehyde + 2,6-dichlorophenol indophenol
-
 Gluconobacter sp. acetate + reduced 2,6-dichlorophenol indophenol
-
 ?
1.2.5.2 acetaldehyde + 2,6-dichlorophenol indophenol
-
 Gluconobacter sp. 33 (DSM Z 3504) acetate + reduced 2,6-dichlorophenol indophenol
-
 ?
1.2.5.2 acetaldehyde + 2,6-dichlorophenol indophenol
-
 Gluconobacter sp. DSM 3504 / ATCC 15163 acetate + reduced 2,6-dichlorophenol indophenol
-
 ?
1.2.5.2 acetaldehyde + phenazine methosulfate
-
 Gluconobacter sp. acetate + reduced phenazine methosulfate
-
 ?
1.2.5.2 acetaldehyde + phenazine methosulfate
-
 Gluconobacter sp. 33 (DSM Z 3504) acetate + reduced phenazine methosulfate
-
 ?
1.2.5.2 acetaldehyde + phenazine methosulfate
-
 Gluconobacter sp. DSM 3504 / ATCC 15163 acetate + reduced phenazine methosulfate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.5 heterotrimer 1 * 80000 + 1 * 50000 + 1 * 15000, SDS-PAGE Gluconobacter sp.
1.2.5.2 heterodimer 1 * 80000 + 1 * 50000, SDS-PAGE Gluconobacter sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.5.5 PQQ-ADH
-
 Gluconobacter sp.
1.1.5.5 PQQ-ADH
-
 Gluconobacter sp. 33
1.1.5.5 pyrroloquinoline quinone-dependent alcohol dehydrogenase
-
 Gluconobacter sp.
1.2.5.2 PQQ-AldDH
-
 Gluconobacter sp.
1.2.5.2 pyrroloquinoline quinone-dependent aldehyde dehydrogenase
-
 Gluconobacter sp.