Literature summary extracted from

Yamanaka, Y.; Zeppieri, L.; Nicolet, Y.; Marinoni, E.N.; de Oliveira, J.S.; Odaka, M.; Dean, D.R.; Fontecilla-Camps, J.C.
Crystal structure and functional studies of an unusual L-cysteine desulfurase from Archaeoglobus fulgidus (2013), Dalton Trans., 42, 3092-3099.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.8.1.7	expressed in Escherichia coli C41 cells	Archaeoglobus fulgidus

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
2.8.1.7	hanging drop vapor diffusion method, using 0.2 M calcium acetate, 0.1 sodium cacodylate pH 6.5, 40% (v/v) PEG 300	Archaeoglobus fulgidus

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.8.1.7	Archaeoglobus fulgidus	O29689	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.8.1.7	ammonium sulfate precipitation, column chromatography, and Superose 12 gel filtration	Archaeoglobus fulgidus

Synonyms

EC Number	Synonyms	Comment	Organism
2.8.1.7	IscS	-	Archaeoglobus fulgidus
2.8.1.7	L-cysteine desulfurase	-	Archaeoglobus fulgidus

Cofactor

EC Number	Cofactor	Comment	Organism	Structure
2.8.1.7	pyridoxal 5'-phosphate	adding pyridoxal 5'-phosphate to IscS produces an enzyme that displays in vitro L-cysteine desulfurase activity	Archaeoglobus fulgidus
2.8.1.7	pyridoxamine 5'-phosphate	the enzyme contains pyridoxamine 5'-phosphate which is catalytically inactive in the cysteine desulfurase reaction, leading to a lack of cysteine desulfurase activity	Archaeoglobus fulgidus

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Release 2023.1 (January 2023)