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Literature summary extracted from

  • Vazquez-Dorado, S.; Sanjuan, A.; Comesana, A.S.; de Carlos, A.
    Identification of octopine dehydrogenase from Mytilus galloprovincialis (2011), Comp. Biochem. Physiol. B, 160, 94-103.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.11 expressed in Escherichia coli BL21 cells Mytilus galloprovincialis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.11 0.024
-
 NADH GST-tagged enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
1.5.1.11 0.026
-
 NADH crude extract enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
1.5.1.11 0.028
-
 NADH recombinant enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
1.5.1.11 0.25
-
 pyruvate crude extract enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
1.5.1.11 0.35
-
 pyruvate GST-tagged enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
1.5.1.11 0.35
-
 pyruvate recombinant enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
1.5.1.11 1.4
-
 L-lysine GST-tagged enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
1.5.1.11 1.8
-
 L-lysine crude extract enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
1.5.1.11 1.8
-
 L-lysine recombinant enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.11 44300
-
 x * 44300, calculated from amino acid sequence Mytilus galloprovincialis
1.5.1.11 70000
-
 gel filtration Mytilus galloprovincialis

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.11 Mytilus galloprovincialis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.11 glutathione Sepharose column chromatography Mytilus galloprovincialis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.1.11 digestive gland
-
 Mytilus galloprovincialis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.5.1.11 0.45
-
 crude extract enzyme, using beta-alanine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
1.5.1.11 1.5
-
 crude extract enzyme, using L-alanine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
1.5.1.11 1.7
-
 crude extract enzyme, using glycine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
1.5.1.11 10.1
-
 recombinant enzyme, using glycine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
1.5.1.11 10.4
-
 crude extract enzyme, using L-arginine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
1.5.1.11 33.3
-
 recombinant enzyme, using L-alanine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis
1.5.1.11 324.8
-
 recombinant enzyme, using L-arginine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication Mytilus galloprovincialis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.11 beta-alanine + pyruvate + NADH + H+
-
 Mytilus galloprovincialis ?
-
 ?
1.5.1.11 glycine + pyruvate + NADH + H+
-
 Mytilus galloprovincialis ?
-
 ?
1.5.1.11 L-alanine + pyruvate + NADH + H+
-
 Mytilus galloprovincialis ?
-
 ?
1.5.1.11 L-arginine + pyruvate + NADH + H+ highest affinity for L-arginine Mytilus galloprovincialis N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O
-
 ?
1.5.1.11 L-lysine + pyruvate + NADH + H+
-
 Mytilus galloprovincialis ?
-
 ?
1.5.1.11 additional information the enzyme shows no activity when taurine or beta-alanine is used as substrate Mytilus galloprovincialis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.11 ? x * 44300, calculated from amino acid sequence Mytilus galloprovincialis
1.5.1.11 ? x * 70000, GST-tagged enzyme, SDS-PAGE Mytilus galloprovincialis

Synonyms

EC Number Synonyms Comment Organism
1.5.1.11 OcDH
-
 Mytilus galloprovincialis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.11 6.2
-
-
 Mytilus galloprovincialis

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.11 NADH
-
 Mytilus galloprovincialis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.5.1.11 Mytilus galloprovincialis calculated from amino acid sequence
-
 5.9