BRENDA - Enzyme Database show

Identification of octopine dehydrogenase from Mytilus galloprovincialis

Vazquez-Dorado, S.; Sanjuan, A.; Comesana, A.S.; de Carlos, A.; Comp. Biochem. Physiol. B 160, 94-103 (2011)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.5.1.11
expressed in Escherichia coli BL21 cells
Mytilus galloprovincialis
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.5.1.11
0.024
-
NADH
GST-tagged enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
0.026
-
NADH
crude extract enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
0.028
-
NADH
recombinant enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
0.25
-
pyruvate
crude extract enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
0.35
-
pyruvate
GST-tagged enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication; recombinant enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
1.4
-
L-lysine
GST-tagged enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
1.8
-
L-lysine
crude extract enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication; recombinant enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.5.1.11
44300
-
x * 44300, calculated from amino acid sequence
Mytilus galloprovincialis
1.5.1.11
70000
-
gel filtration
Mytilus galloprovincialis
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.5.1.11
Mytilus galloprovincialis
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.5.1.11
glutathione Sepharose column chromatography
Mytilus galloprovincialis
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.5.1.11
digestive gland
-
Mytilus galloprovincialis
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.5.1.11
0.45
-
crude extract enzyme, using beta-alanine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
1.5
-
crude extract enzyme, using L-alanine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
1.7
-
crude extract enzyme, using glycine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
10.1
-
recombinant enzyme, using glycine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
10.4
-
crude extract enzyme, using L-arginine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
33.3
-
recombinant enzyme, using L-alanine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
324.8
-
recombinant enzyme, using L-arginine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.5.1.11
beta-alanine + pyruvate + NADH + H+
-
724787
Mytilus galloprovincialis
?
-
-
-
?
1.5.1.11
glycine + pyruvate + NADH + H+
-
724787
Mytilus galloprovincialis
?
-
-
-
?
1.5.1.11
L-alanine + pyruvate + NADH + H+
-
724787
Mytilus galloprovincialis
?
-
-
-
?
1.5.1.11
L-arginine + pyruvate + NADH + H+
highest affinity for L-arginine
724787
Mytilus galloprovincialis
N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O
-
-
-
?
1.5.1.11
L-lysine + pyruvate + NADH + H+
-
724787
Mytilus galloprovincialis
?
-
-
-
?
1.5.1.11
additional information
the enzyme shows no activity when taurine or beta-alanine is used as substrate
724787
Mytilus galloprovincialis
?
-
-
-
-
Subunits
EC Number
Subunits
Commentary
Organism
1.5.1.11
?
x * 44300, calculated from amino acid sequence; x * 70000, GST-tagged enzyme, SDS-PAGE
Mytilus galloprovincialis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.5.1.11
6.2
-
-
Mytilus galloprovincialis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.5.1.11
NADH
-
Mytilus galloprovincialis
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.5.1.11
Mytilus galloprovincialis
calculated from amino acid sequence
-
5.9
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.5.1.11
expressed in Escherichia coli BL21 cells
Mytilus galloprovincialis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.5.1.11
NADH
-
Mytilus galloprovincialis
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.5.1.11
0.024
-
NADH
GST-tagged enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
0.026
-
NADH
crude extract enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
0.028
-
NADH
recombinant enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
0.25
-
pyruvate
crude extract enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
0.35
-
pyruvate
GST-tagged enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication; recombinant enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
1.4
-
L-lysine
GST-tagged enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
1.8
-
L-lysine
crude extract enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication; recombinant enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.5.1.11
44300
-
x * 44300, calculated from amino acid sequence
Mytilus galloprovincialis
1.5.1.11
70000
-
gel filtration
Mytilus galloprovincialis
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.5.1.11
glutathione Sepharose column chromatography
Mytilus galloprovincialis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.5.1.11
digestive gland
-
Mytilus galloprovincialis
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
1.5.1.11
0.45
-
crude extract enzyme, using beta-alanine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
1.5
-
crude extract enzyme, using L-alanine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
1.7
-
crude extract enzyme, using glycine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
10.1
-
recombinant enzyme, using glycine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
10.4
-
crude extract enzyme, using L-arginine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
33.3
-
recombinant enzyme, using L-alanine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
1.5.1.11
324.8
-
recombinant enzyme, using L-arginine as substrate, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
Mytilus galloprovincialis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.5.1.11
beta-alanine + pyruvate + NADH + H+
-
724787
Mytilus galloprovincialis
?
-
-
-
?
1.5.1.11
glycine + pyruvate + NADH + H+
-
724787
Mytilus galloprovincialis
?
-
-
-
?
1.5.1.11
L-alanine + pyruvate + NADH + H+
-
724787
Mytilus galloprovincialis
?
-
-
-
?
1.5.1.11
L-arginine + pyruvate + NADH + H+
highest affinity for L-arginine
724787
Mytilus galloprovincialis
N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O
-
-
-
?
1.5.1.11
L-lysine + pyruvate + NADH + H+
-
724787
Mytilus galloprovincialis
?
-
-
-
?
1.5.1.11
additional information
the enzyme shows no activity when taurine or beta-alanine is used as substrate
724787
Mytilus galloprovincialis
?
-
-
-
-
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.5.1.11
?
x * 44300, calculated from amino acid sequence; x * 70000, GST-tagged enzyme, SDS-PAGE
Mytilus galloprovincialis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.5.1.11
6.2
-
-
Mytilus galloprovincialis
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.5.1.11
Mytilus galloprovincialis
calculated from amino acid sequence
-
5.9