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Literature summary extracted from

  • Liu, H.; Sun, G.; Ma, X.; Sun, L.; Lu, X.; Zhang, P.
    Purification and characterization of a sarcosine oxidase from Bacillus sp. BSD-8 (2010), Chin. J. Biotechnol., 26, 335-340.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.5.3.1 analysis sarcosine oxidases can be applied in enzymatic measuring of creatinine Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.3.1 Ag+ strong inhibition Bacillus sp. (in: Bacteria)
1.5.3.1 Hg2+ strong inhibition Bacillus sp. (in: Bacteria)
1.5.3.1 additional information no inhibition by Tween 20 and Triton X-100 Bacillus sp. (in: Bacteria)
1.5.3.1 SDS strong inhibition Bacillus sp. (in: Bacteria)
1.5.3.1 Tween 80 strong inhibition Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.1 3.1
-
 sarcosine pH 8.5, 60°C Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.3.1 51000
-
 x * 51000 Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.3.1 sarcosine + H2O + O2 Bacillus sp. (in: Bacteria)
-
 glycine + formaldehyde + H2O2
-
 ?
1.5.3.1 sarcosine + H2O + O2 Bacillus sp. (in: Bacteria) BSD-8
-
 glycine + formaldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.1 Bacillus sp. (in: Bacteria)
-
 isolated from soil
-
1.5.3.1 Bacillus sp. (in: Bacteria) BSD-8
-
 isolated from soil
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.1 native enzyme by ammoniumm sulfate fractionation, hydrophobic interaction and anion exchange chromatography, and gel filtration Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.1 sarcosine + H2O + O2
-
 Bacillus sp. (in: Bacteria) glycine + formaldehyde + H2O2
-
 ?
1.5.3.1 sarcosine + H2O + O2
-
 Bacillus sp. (in: Bacteria) BSD-8 glycine + formaldehyde + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.1 ? x * 51000 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.3.1 60
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.5.3.1 60
-
 purified enzyme, stable at Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.1 8.5
-
-
 Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.5.3.1 8 10 purified enzyme, stable at Bacillus sp. (in: Bacteria)

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.3.1 flavin flavoenzyme with noncovalently bound flavin Bacillus sp. (in: Bacteria)