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Literature summary extracted from

  • Wilding, B.; Winkler, M.; Petschacher, B.; Kratzer, R.; Egger, S.; Steinkellner, G.; Lyskowski, A.; Nidetzky, B.; Gruber, K.; Klempier, N.
    Targeting the substrate binding site of E. coli nitrile reductase QueF by modeling, substrate and enzyme engineering (2013), Chemistry, 19, 7007-7012.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.13
-
 Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.7.1.13 construction of homology model and docking studies of natural and non-natural substrates. Residues C190 and D197 play an essential role in the catalytic mechanism Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.7.1.13 C190A complete loss of activity Escherichia coli
1.7.1.13 D197N complete loss of activity Escherichia coli
1.7.1.13 E230Q about 4% of wild-type activity for natural substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one Escherichia coli
1.7.1.13 F228W about 8% of wild-type activity for natural substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one Escherichia coli
1.7.1.13 H229A about 15% of wild-type activity for natural substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one Escherichia coli
1.7.1.13 Q89A poor activity Escherichia coli
1.7.1.13 Q89L poor activity Escherichia coli
1.7.1.13 S90A about 10% of wild-type activity for natural substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.13 0.006
-
 NADPH wild-type, pH 7.5, 25°C Escherichia coli
1.7.1.13 0.0061
-
 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one wild-type, pH 7.5, 25°C Escherichia coli
1.7.1.13 0.176
-
 5-cyanopyrrolo[2,3-d]pyrimidin-4-one wild-type, pH 7.5, 25°C Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.1.13 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one + 2 NADPH + 2 H+ Escherichia coli natural substrate 2-amino-5-amino-methyl-pyrrolo[2,3-d]pyrimidin-4-one + 2 NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.13 Escherichia coli Q46920
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.13 3
-
 substrate 5-cyanopyrrolo[2,3-d]pyrimidin-4-one, mutant E230Q, pH 7.5, 25°C Escherichia coli
1.7.1.13 3.8
-
 substrate 5-cyanopyrrolo[2,3-d]pyrimidin-4-one, mutant S90A, pH 7.5, 25°C Escherichia coli
1.7.1.13 4.4
-
 substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one, mutant E230Q, pH 7.5, 25°C Escherichia coli
1.7.1.13 8.7
-
 substrate 5-cyanopyrrolo[2,3-d]pyrimidin-4-one, mutant F228W, pH 7.5, 25°C Escherichia coli
1.7.1.13 10.8
-
 substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one, mutant F228W, pH 7.5, 25°C Escherichia coli
1.7.1.13 12.9
-
 substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one, mutant S90A, pH 7.5, 25°C Escherichia coli
1.7.1.13 14.3
-
 substrate 5-cyanopyrrolo[2,3-d]pyrimidin-4-one, mutant H229A, pH 7.5, 25°C Escherichia coli
1.7.1.13 17.9
-
 substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one, mutant H229A, pH 7.5, 25°C Escherichia coli
1.7.1.13 90
-
 substrate 5-cyanopyrrolo[2,3-d]pyrimidin-4-one, wild-type, pH 7.5, 25°C Escherichia coli
1.7.1.13 117
-
 substrate 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one, wild-type, pH 7.5, 25°C Escherichia coli

Storage Stability

EC Number Storage Stability Organism
1.7.1.13 -70°C, 1 year, 1% residual activity Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.13 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one + 2 NADPH + 2 H+ natural substrate Escherichia coli 2-amino-5-amino-methyl-pyrrolo[2,3-d]pyrimidin-4-one + 2 NADP+
-
 ?
1.7.1.13 2-amino-5-cyanopyrrolo[2,3-d]pyrimidine + 2 NADPH + 2 H+ analogue of natural substrate, poor substrate Escherichia coli 2-amino-5-amino-methyl-pyrrolo[2,3-d]pyrimidine + 2 NADP+
-
 ?
1.7.1.13 5-cyanopyrrolo[2,3-d]pyrimidin-4-one + 2 NADPH + 2 H+ analogue of natural substrate Escherichia coli 5-amino-methyl-pyrrolo[2,3-d]pyrimidin-4-one + 2 NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.1.13 QueF
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.1.13 0.05
-
 5-cyanopyrrolo[2,3-d]pyrimidin-4-one wild-type, pH 7.5, 25°C Escherichia coli
1.7.1.13 0.11
-
 2-amino-5-cyanopyrrolo[2,3-d]pyrimidin-4-one wild-type, pH 7.5, 25°C Escherichia coli
1.7.1.13 0.142
-
 NADPH wild-type, pH 7.5, 25°C Escherichia coli