Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bekhouche, M.; Blum, L.; Doumeche, B.
    Ionic liquid-inspired cations covalently bound to formate dehydrogenase improve its stability and activity in ionic liquids (2011), ChemCatChem, 3, 875-882.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.17.1.9 1-methyl-3-methylimidazolium dimethylphosphate adding more than 40% (v/v) of 1-methyl-3-methylimidazolium dimethylphosphate inactivates formate dehydrogenase. In the presence of 30% (v/v) 1-methyl-3-methylimidazolium dimethylphosphate, the catalytic efficiency of the wild type enzyme is reduced by 2.8fold [Candida] boidinii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.17.1.9 0.043
-
 NAD+ wild type enzyme, in the absence of 1-methyl-3-methylimidazolium dimethylphosphate, in 50 mM carbonate buffer, pH 9.7, at 30°C [Candida] boidinii
1.17.1.9 0.177
-
 NAD+ wild type enzyme, in the presence of 30% (v/v) 1-methyl-3-methylimidazolium dimethylphosphate, in 50 mM carbonate buffer, pH 9.7, at 30°C [Candida] boidinii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.17.1.9 formate + NAD+ [Candida] boidinii
-
 CO2 + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.17.1.9 [Candida] boidinii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.17.1.9 formate + NAD+
-
 [Candida] boidinii CO2 + NADH + H+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.17.1.9 FDH
-
 [Candida] boidinii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.17.1.9 890
-
 NAD+ wild type enzyme, in the absence of 1-methyl-3-methylimidazolium dimethylphosphate, in 50 mM carbonate buffer, pH 9.7, at 30°C [Candida] boidinii
1.17.1.9 1300
-
 NAD+ wild type enzyme, in the presence of 30% (v/v) 1-methyl-3-methylimidazolium dimethylphosphate, in 50 mM carbonate buffer, pH 9.7, at 30°C [Candida] boidinii

Cofactor

EC Number Cofactor Comment Organism Structure
1.17.1.9 NAD+
-
 [Candida] boidinii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.17.1.9 7333
-
 NAD+ wild type enzyme, in the presence of 30% (v/v) 1-methyl-3-methylimidazolium dimethylphosphate, in 50 mM carbonate buffer, pH 9.7, at 30°C [Candida] boidinii
1.17.1.9 20600
-
 NAD+ wild type enzyme, in the absence of 1-methyl-3-methylimidazolium dimethylphosphate, in 50 mM carbonate buffer, pH 9.7, at 30°C [Candida] boidinii