Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Hernandez-Ortega, A.; Ferreira, P.; Merino, P.; Medina, M.; Guallar, V.; Martinez, A.T.
    Stereoselective hydride transfer by aryl-alcohol oxidase, a member of the GMC superfamily (2012), ChemBioChem, 13, 427-435.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.1.3.7 F501A the AAO preference for (S)-1-(4-fluorophenyl)ethanol is increased threefold when the bulky side chain of Phe501 is removed in the F501A variant, which shows a stereoselectivity S/R ratio of 66:1 for this secondary alcohol Pleurotus eryngii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.7 additional information
-
 additional information steady state and transient state kinetic constants for alcohol and O2 of AAO oxidation of a deuterated and normal (alpha-protiated) 4-methoxybenzyl alcohol, solvent kinetic isotope effects, overview Pleurotus eryngii
1.1.3.7 0.025
-
 4-methoxybenzyl alcohol substrate alpha-deuterated 4-methoxybenzyl alcohol, pH 6.0, 25°C Pleurotus eryngii
1.1.3.7 0.049
-
 4-methoxybenzyl alcohol substrate 4-methoxybenzyl alcohol, pH 6.0, 25°C Pleurotus eryngii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.3.7 (R,S)-4-methoxybenzyl alcohol + O2 Pleurotus eryngii over 98% excess of the R enantiomer after treatment of racemic 1-(4-methoxyphenyl)ethanol, the hydride transfer is highly stereoselective 1-(4-methoxyphenyl)ethanol + H2O2
-
 ?
1.1.3.7 4-methoxybenzyl alcohol + O2 Pleurotus eryngii
-
 4-methoxybenzaldehyde + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.7 Pleurotus eryngii O94219
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.3.7 an aromatic primary alcohol + O2 = an aromatic aldehyde + H2O2 oxidation mechanism by AAO, overview Pleurotus eryngii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.7 (R,S)-4-methoxybenzyl alcohol + O2 over 98% excess of the R enantiomer after treatment of racemic 1-(4-methoxyphenyl)ethanol, the hydride transfer is highly stereoselective Pleurotus eryngii 1-(4-methoxyphenyl)ethanol + H2O2
-
 ?
1.1.3.7 (S)-1-(4-fluorophenyl)ethanol + O2 mutant F501A Pleurotus eryngii 1-(4-fluorophenyl)acetaldehyde + H2O2
-
 ?
1.1.3.7 4-methoxybenzyl alcohol + O2
-
 Pleurotus eryngii 4-methoxybenzaldehyde + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.3.7 AAO
-
 Pleurotus eryngii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.7 25
-
 assay at Pleurotus eryngii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.3.7 25
-
 4-methoxybenzyl alcohol substrate alpha-deuterated 4-methoxybenzyl alcohol, pH 6.0, 25°C Pleurotus eryngii
1.1.3.7 196
-
 4-methoxybenzyl alcohol substrate 4-methoxybenzyl alcohol, pH 6.0, 25°C Pleurotus eryngii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.7 6
-
 assay at Pleurotus eryngii

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.7 FAD
-
 Pleurotus eryngii

General Information

EC Number General Information Comment Organism
1.1.3.7 evolution the enzyme belongs to the glucose methanol choline oxidase superfamily Pleurotus eryngii
1.1.3.7 additional information docking of 4-methoxybenzyl alcohol at the buried crystal active site, and quantum mechanical/molecular mechanical study, overview Pleurotus eryngii
1.1.3.7 physiological function the enzyme provides H2O2 to ligninolytic peroxidases Pleurotus eryngii

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.3.7 1020
-
 4-methoxybenzyl alcohol substrate alpha-deuterated 4-methoxybenzyl alcohol, pH 6.0, 25°C Pleurotus eryngii
1.1.3.7 3980
-
 4-methoxybenzyl alcohol substrate 4-methoxybenzyl alcohol, pH 6.0, 25°C Pleurotus eryngii