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Literature summary extracted from

  • Staab, C.A.; Hartmanova, T.; El-Hawari, Y.; Ebert, B.; Kisiela, M.; Wsol, V.; Martin, H.J.; Maser, E.
    Studies on reduction of S-nitrosoglutathione by human carbonyl reductases 1 and 3 (2011), Chem. Biol. Interact., 191, 95-103.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.184
-
 Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.184 C227S displays a similar kcat, but a 30fold higher Km value for S-nitrosoglutathione, and does not show substrate inhibition Homo sapiens
1.1.1.184 D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P mutations introduce activity towards S-nitrosoglutathione Homo sapiens
1.1.1.184 P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P mutations introduce activity towards S-nitrosoglutathione Homo sapiens
1.1.1.184 Q142M/C143S/P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P/H270S mutations introduce activity towards S-nitrosoglutathione Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.184 S-nitrosoglutathione substrate inhibition above 5 * Km, enzyme is covalantly modified. Treatment with dithiothreitol, but not with ascorbic acid, rescues the activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.184 0.0128
-
 9,10-phenanthrenequinone mutant C227S, pH 7.4, temperature not specified in the publication Homo sapiens
1.1.1.184 0.028
-
 S-nitrosoglutathione wild-type, pH 7.4, temperature not specified in the publication Homo sapiens
1.1.1.184 0.0354
-
 9,10-phenanthrenequinone wild-type, pH 7.4, temperature not specified in the publication Homo sapiens
1.1.1.184 0.81
-
 S-nitrosoglutathione mutant C227S, pH 7.4, temperature not specified in the publication Homo sapiens
1.1.1.184 0.832
-
 S-nitrosoglutathione mutant Q142M/C143S/P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P/H270S, pH 7.4, temperature not specified in the publication Homo sapiens
1.1.1.184 1.09
-
 S-nitrosoglutathione mutant P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244PpH 7.4, temperature not specified in the publication Homo sapiens
1.1.1.184 2.93
-
 S-nitrosoglutathione mutant D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P, pH 7.4, temperature not specified in the publication Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.184 Homo sapiens O75828 isoform CBR 3
-
1.1.1.184 Homo sapiens P16152 isoform CBR 1
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.184 9,10-phenanthrenequinone + NADPH + H+
-
 Homo sapiens ? + NADP+
-
 ?
1.1.1.184 S-nitrosoglutathione + NADPH + H+
-
 Homo sapiens ? + NADP+
-
 ?
1.1.1.184 S-nitrosoglutathione + NADPH + H+ no substrate for wild-type, but substrate for mutants D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P, P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P, and Q142M/C143S/P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P/H270S Homo sapiens ? + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.184 CBR 1
-
 Homo sapiens
1.1.1.184 CBR 3
-
 Homo sapiens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.184 2.02
-
 S-nitrosoglutathione mutant C227S, pH 7.4, temperature not specified in the publication Homo sapiens
1.1.1.184 2.62
-
 S-nitrosoglutathione wild-type, pH 7.4, temperature not specified in the publication Homo sapiens
1.1.1.184 5.05
-
 S-nitrosoglutathione mutant D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P, pH 7.4, temperature not specified in the publication Homo sapiens
1.1.1.184 5.25
-
 9,10-phenanthrenequinone wild-type, pH 7.4, temperature not specified in the publication Homo sapiens
1.1.1.184 10.83
-
 S-nitrosoglutathione mutant P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244PpH 7.4, temperature not specified in the publication Homo sapiens
1.1.1.184 20.45
-
 S-nitrosoglutathione mutant Q142M/C143S/P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P/H270S, pH 7.4, temperature not specified in the publication Homo sapiens
1.1.1.184 36.2
-
 9,10-phenanthrenequinone mutant C227S, pH 7.4, temperature not specified in the publication Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.184 NADPH
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.184 0.67
-
 S-nitrosoglutathione wild-type, pH 7.4, temperature not specified in the publication Homo sapiens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.184 1.72
-
 S-nitrosoglutathione mutant D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P, pH 7.4, temperature not specified in the publication Homo sapiens
1.1.1.184 2.5
-
 S-nitrosoglutathione mutant C227S, pH 7.4, temperature not specified in the publication Homo sapiens
1.1.1.184 9.93
-
 S-nitrosoglutathione mutant P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244PpH 7.4, temperature not specified in the publication Homo sapiens
1.1.1.184 24.58
-
 S-nitrosoglutathione mutant Q142M/C143S/P230W/D236A/K238P/D239K/S240A/I241T/R242K/T243S/V244P/H270S, pH 7.4, temperature not specified in the publication Homo sapiens
1.1.1.184 93.45
-
 S-nitrosoglutathione wild-type, pH 7.4, temperature not specified in the publication Homo sapiens
1.1.1.184 148.3
-
 9,10-phenanthrenequinone wild-type, pH 7.4, temperature not specified in the publication Homo sapiens
1.1.1.184 2828
-
 9,10-phenanthrenequinone mutant C227S, pH 7.4, temperature not specified in the publication Homo sapiens