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Literature summary extracted from

  • Strickland, K.C.; Krupenko, N.I.; Dubard, M.E.; Hu, C.J.; Tsybovsky, Y.; Krupenko, S.A.
    Enzymatic properties of ALDH1L2, a mitochondrial 10-formyltetrahydrofolate dehydrogenase (2011), Chem. Biol. Interact., 191, 129-136.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.5.1.6 4'-phosphopantetheinyl transferase 4'-phosphopantetheinyl transferase activates dehydrogenase catalysis of ALDH1L2 by modifying Ser375 Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.6 expressed in Escherichia coli BL21(DE3) Codon Plus cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.6 S374A the mutant is activated by 4'-phosphopantetheinyl transferase Homo sapiens
1.5.1.6 S374A/S375A the mutant is not activated by 4'-phosphopantetheinyl transferase Homo sapiens
1.5.1.6 S375A the mutant is not activated by 4'-phosphopantetheinyl transferase Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.6 0.0114
-
 10-formyltetrahydrofolate at pH 7.0 and 30°C Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.1.6 mitochondrion
-
 Homo sapiens 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.5.1.6 14500
-
 4 * 14500, C-terminal domain, SDS-PAGE Homo sapiens
1.5.1.6 58000
-
 C-terminal domain, gel filtration Homo sapiens
1.5.1.6 100000
-
 full-length enzyme, gel filtration Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O Homo sapiens
-
 tetrahydrofolate + CO2 + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.6 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.5.1.6 additional information S375 is the putative site of enzyme phosphopantetheinylation Homo sapiens

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.6
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.6 10-formyltetrahydrofolate + NADP+ + H2O
-
 Homo sapiens tetrahydrofolate + CO2 + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.1.6 homotetramer 4 * 14500, C-terminal domain, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
1.5.1.6 10-fTHF dehydrogenase
-
 Homo sapiens
1.5.1.6 ALDH1L2
-
 Homo sapiens
1.5.1.6 mtFDH mitochondrial isoform Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.6 NADP+
-
 Homo sapiens