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Literature summary extracted from

  • Dai, Y.; Huang, C.; Wen, L.; Sheu, D.; Lin, C.
    Lemon ascorbate peroxidase: cDNA cloning and biochemical characterization (2012), Bot. Stud., 53, 9-17.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.11 expression in Saccharomyces cerevisiae Citrus limon

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.11.1.11 construction of a 3-D structural model based on the crystal structure of Pisum sativum Apx, PDB code 1APX Citrus limon

General Stability

EC Number General Stability Organism
1.11.1.11 enzyme retains 35% activity after chymotrypsin digestion at pH 8 and 37°C for 40 min Citrus limon

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.11 imidazole enzyme shows a decrease in its activity with increasing imidazole concentration, approximately 50% activity is lost in the presence of 0.8 M imidazole Citrus limon

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.11 0.11
-
 H2O2 pH 7.4, 37°C Citrus limon
1.11.1.11 0.4
-
 L-ascorbate pH 7.4, 37°C Citrus limon

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.11 28100
-
 ESI Q-TOF Citrus limon
1.11.1.11 29000
-
 1 * 29000, calculated, 1 * 29000, SDS-PAGE, His-tagged recombinant protein Citrus limon

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.11 Citrus limon J7EIR8
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.11 2 L-ascorbate + H2O2 + 2 H+
-
 Citrus limon 2 monodehydroascorbate + 2 H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.11 monomer 1 * 29000, calculated, 1 * 29000, SDS-PAGE, His-tagged recombinant protein Citrus limon

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.11 45
-
 half-life 6.5 min Citrus limon

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.11 71.5
-
 L-ascorbate pH 7.4, 37°C Citrus limon
1.11.1.11 84.5
-
 H2O2 pH 7.4, 37°C Citrus limon

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.11 6 8
-
 Citrus limon

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.11 Citrus limon calculated
-
 5.6

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.11 178
-
 L-ascorbate pH 7.4, 37°C Citrus limon
1.11.1.11 768
-
 H2O2 pH 7.4, 37°C Citrus limon