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Literature summary extracted from

  • Reiss, R.; Ihssen, J.; Thoeny-Meyer, L.
    Bacillus pumilus laccase: a heat stable enzyme with a wide substrate spectrum (2011), BMC Biotechnol., 11, 9-9.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.10.3.2 synthesis owing to their broad substrate range laccases are considered to be versatile biocatalysts which are capable of oxidizing natural and non-natural industrial compounds, with water as sole by-product Bacillus pumilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 CotA-type laccase, DNA and amino acid sequence determination and analysis, recombinant expression in Escherichia coli strain JM109 Bacillus pumilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 additional information
-
 additional information kinetics do not fit a Michaelis-Menten equation Bacillus pumilus
1.10.3.2 0.08
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.0, 22°C, recombinant enzyme Bacillus pumilus
1.10.3.2 0.68
-
 2,6-dimethoxyphenol pH 7.0, 22°C, recombinant enzyme Bacillus pumilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ the CotA-type laccase is associated with 4 Cu2+ ions, content determined by inductive coupled plasma optical emission spectroscopy Bacillus pumilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 58000
-
 x * 58000, recombinant CotA-type laccase, SDS-PAGE Bacillus pumilus

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Bacillus pumilus
-
 gene cotA
-
1.10.3.2 Bacillus pumilus DSM 27
-
 gene cotA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 recombinant CotA-type laccase from Escherichia coli strain JM109 by anion exchange chromatography, ultrafiltration, and gel filtration Bacillus pumilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 (2E)-3-(3,4-dihydroxyphenyl)prop-2-enoic acid + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 (2E)-3-(4-hydroxy-3-methoxyphenyl)prop-2-enoic acid + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 (2Z,2'Z)-2,2'-(1Z,2Z)-hydrazine-1,2-diylidenebis(3-ethyl-2,3-dihydro-1,3-benzothiazole-6-sulfonic acid) + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 1-(2,4,5-trihydroxyphenyl)butan-1-one + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 1-(4-hydroxy-3,5-dimethoxyphenyl)ethanone + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Bacillus pumilus DSM 27 ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Bacillus pumilus DSM 27 ?
-
 ?
1.10.3.2 3,4,5-trihydroxybenzoic acid + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 3,4,5-trihydroxybenzoic acid + O2
-
 Bacillus pumilus DSM 27 ?
-
 ?
1.10.3.2 3,4-dihydroxybenzoic acid + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 3,4-dihydroxybenzoic acid + O2
-
 Bacillus pumilus DSM 27 ?
-
 ?
1.10.3.2 3-amino-4-hydroxybenzoic acid + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 3-hydroxy-L-tyrosine + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 4-(2-aminoethyl)benzene-1,2-diol + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 4-(hydroxymethyl)-2-methoxyphenol + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 4-amino-3-hydroxybenzoic acid + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 4-hydroxy-3,5-dimethoxybenzaldehyde + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 4-hydroxy-3,5-dimethoxybenzoic acid + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 4-methylbenzene-1,2-diol + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 5-(hydroxymethyl)-2-methoxyphenol + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 benzene-1,2,3-triol + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 benzene-1,2-diol + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Bacillus pumilus DSM 27 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 58000, recombinant CotA-type laccase, SDS-PAGE Bacillus pumilus

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 CotA-type laccase
-
 Bacillus pumilus
1.10.3.2 spore coat protein A
-
 Bacillus pumilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 70
-
-
 Bacillus pumilus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 20 75 activity range Bacillus pumilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 4.9
-
 syringaldazine pH 6.5, 22°C, recombinant enzyme, with 0.8% v/v DMSO Bacillus pumilus
1.10.3.2 11
-
 2,6-dimethoxyphenol pH 7.0, 22°C, recombinant enzyme Bacillus pumilus
1.10.3.2 66
-
 syringaldazine pH 7.0, 22°C, recombinant enzyme, with 25% v/v DMSO Bacillus pumilus
1.10.3.2 291
-
 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.0, 22°C, recombinant enzyme Bacillus pumilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 4 7 broad optimum, dependent on the substrate Bacillus pumilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 2 8 activity range Bacillus pumilus