EC Number | Application | Comment | Organism |
---|---|---|---|
1.1.1.289 | synthesis | usage of a substrate-coupled biocatalytic process driven by an NADPH-dependent sorbose reductase from Candida albicans for the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate | Candida albicans |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.1.1.289 | expression in Escherichia coli strain Rosseta (DE3) | Candida albicans |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.289 | D-sorbitol + NADP+ | Candida albicans | - |
L-sorbose + NADPH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.1.1.289 | Candida albicans | - |
- |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.1.1.289 | D-mannitol + NADP+ | - |
Candida albicans | D-mannose + NADPH + H+ | - |
r | |
1.1.1.289 | D-sorbitol + NADP+ | - |
Candida albicans | L-sorbose + NADPH + H+ | - |
r | |
1.1.1.289 | D-xylitol + NADP+ | - |
Candida albicans | D-xylose + NADPH + H+ | - |
r | |
1.1.1.289 | additional information | development of a substrate-coupled biocatalytic process driven by NADPH-dependent sorbose reductase for the asymmetric reduction of ethyl 4-chloro-3-oxobutanoate, overview. Ethyl 4-chloro-3-oxobutanoate is reduced to (S)-4-chloro-3-hydroxybutanoate, while NADPH is regenerated by the enzyme via oxidation of sorbitol, mannitol, or xylitol in crude extract of recombinant Escherichia coli cells, overview | Candida albicans | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.1.1.289 | NADPH-dependent sorbose reductase | - |
Candida albicans |
1.1.1.289 | SOU1 | - |
Candida albicans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.1.1.289 | 30 | - |
assay at | Candida albicans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.1.1.289 | 6.2 | - |
assay at | Candida albicans |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.1.1.289 | NADPH | dependent on | Candida albicans |