Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Bao, X.; Liu, A.; Lu, X.; Li, J.J.
    Direct over-expression, characterization and H2O2 stability study of active Pleurotus eryngii versatile peroxidase in Escherichia coli (2012), Biotechnol. Lett., 34, 1537-1543.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.16 expression in Escherichia coli fused to a thioredoxin-hexahistidine tag. Activity of the enzyme increases after removing the tag Pleurotus eryngii

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.16 H2O2 6.4 mM, 5% loss of activity Pleurotus eryngii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.16 0.0007
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.0048
-
 Reactive Black 5 pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 0.078
-
 Mn2+ pH 5.0, 25°C Pleurotus eryngii
1.11.1.16 4.09
-
 veratryl alcohol pH 3.0, 25°C Pleurotus eryngii

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.16 Pleurotus eryngii O94753 isoform Vpl2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.16 recombinant protein Pleurotus eryngii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.16 2.2
-
 substrate Reactive Black 5, pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 7.2
-
 substrate veratryl alcohol, pH 3.0, 25°C Pleurotus eryngii
1.11.1.16 8.8
-
 substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 194
-
 substrate Mn2+, pH 5.0, 25°C Pleurotus eryngii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.16 2 Mn2+ + 2 H+ + H2O2
-
 Pleurotus eryngii 2 Mn3+ + 2 H2O
-
 ?
1.11.1.16 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2
-
 Pleurotus eryngii ? + H2O
-
 ?
1.11.1.16 Reactive Black 5 + H2O2
-
 Pleurotus eryngii oxidized Reactive Black 5 + H2O
-
 ?
1.11.1.16 veratryl alcohol + H2O2 + H+
-
 Pleurotus eryngii veratraldehyde + H2O
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.16 2.7
-
 Reactive Black 5 pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 5.4
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.5, 25°C Pleurotus eryngii
1.11.1.16 6.4
-
 veratryl alcohol pH 3.0, 25°C Pleurotus eryngii
1.11.1.16 138
-
 Mn2+ pH 5.0, 25°C Pleurotus eryngii