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Literature summary extracted from

  • Klose, H.; Röder, J.; Girfoglio, M.; Fischer, R.; Commandeur, U.
    Hyperthermophilic endoglucanase for in planta lignocellulose conversion (2012), Biotechnol. Biofuels, 5, 63.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.4 biofuel production the enzyme is a tool for biomass conversion. The recombinant enzyme acts in high concentrations of ionic liquids and can therefore degrade alpha-cellulose or even complex cell wall preparations under those pretreatment conditions. The enzymatic conversion of lignocellulosic plant biomass into fermentable sugars is a crucial step in the production of biofuels Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in tobacco leaf, including ER-tag Saccharolobus solfataricus
3.2.1.4 expression of SSO1354 in Nicotiana tabacum Saccharolobus solfataricus

General Stability

EC Number General Stability Organism
3.2.1.4 the enzyme tolerates high salt concentrations, with 49.5% residual activity in the presence of 2.4 M NaCl, and 46.5% residual activity in the presence of 2.4 M CaCl2 Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.4 CaCl2 46.5% residual activity in the presence of 2.4 M CaCl2 Saccharolobus solfataricus
3.2.1.4 NaCl 49.5% residual activity in the presence of 2.4 M NaCl Saccharolobus solfataricus

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
3.2.1.4 Acetone 15% (v/v), 49% loss of activity, nearly complete inhibition at 30% (v/v) Saccharolobus solfataricus
3.2.1.4 Acetone 15% v/v, 51% residual activity Saccharolobus solfataricus
3.2.1.4 dimethyl sulfoxide 52% (v/v), 21% loss of activity Saccharolobus solfataricus
3.2.1.4 DMSO 15% v/v, 75% residual activity Saccharolobus solfataricus
3.2.1.4 Ethanol 15% v/v, 48% residual activity Saccharolobus solfataricus
3.2.1.4 Ethanol 15% (v/v), 25% loss of activity Saccharolobus solfataricus
3.2.1.4 Methanol 15% (v/v), 21% loss of activity, nearly complete inhibition at 30% (v/v) Saccharolobus solfataricus
3.2.1.4 Methanol 15% v/v, 79% residual activity Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Saccharolobus solfataricus Q97YG7
-
-
3.2.1.4 Saccharolobus solfataricus P2 Q97YG7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Saccharolobus solfataricus
3.2.1.4 recombinant protein Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 4-methylumbelliferyl beta-D-cellobioside + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.2.1.4 barley beta-D-glucan + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.2.1.4 barley beta-glucan + H2O 136% of the activity with carboxymethyl cellulose Saccharolobus solfataricus ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O
-
 Saccharolobus solfataricus P2 ?
-
 ?
3.2.1.4 lichenan + H2O
-
 Saccharolobus solfataricus ?
-
 ?
3.2.1.4 lichenan + H2O 130% of the activity with carboxymethyl cellulose Saccharolobus solfataricus ?
-
 ?
3.2.1.4 lichenan + H2O
-
 Saccharolobus solfataricus P2 ?
-
 ?
3.2.1.4 lichenan + H2O 130% of the activity with carboxymethyl cellulose Saccharolobus solfataricus P2 ?
-
 ?
3.2.1.4 additional information the enzyme has a beta-1,4 hydrolytic mode of action. It shows no significant activity on PCM3/pachyman, microcrystalline cellulose, or on oat xylan. Concentrated solutions (80–90% v/v) of ionic liquids such as 1-ethyl-3-methylimidazolium acetate and 1,3-dimethylimidazolium dimethylphosphate are able to dissolve crystalline cellulose like AvicelW and cell wall polysaccharides, making them more accessible to hydrolytic enzymes Saccharolobus solfataricus ?
-
 ?
3.2.1.4 additional information no substrates: PCM3/pachyman, avicel, oat xylan Saccharolobus solfataricus ?
-
 ?
3.2.1.4 additional information the enzyme has a beta-1,4 hydrolytic mode of action. It shows no significant activity on PCM3/pachyman, microcrystalline cellulose, or on oat xylan. Concentrated solutions (80–90% v/v) of ionic liquids such as 1-ethyl-3-methylimidazolium acetate and 1,3-dimethylimidazolium dimethylphosphate are able to dissolve crystalline cellulose like AvicelW and cell wall polysaccharides, making them more accessible to hydrolytic enzymes Saccharolobus solfataricus P2 ?
-
 ?
3.2.1.4 additional information no substrates: PCM3/pachyman, avicel, oat xylan Saccharolobus solfataricus P2 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 SSO1354
-
 Saccharolobus solfataricus
3.2.1.4 SSO1354 locus name Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 90
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.4 70
-
 24% residual activity Saccharolobus solfataricus
3.2.1.4 75 100 75°C: 60% of maximal activity, 100°C: about 80% of maximal activity Saccharolobus solfataricus
3.2.1.4 99
-
 79% residual activity Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 90
-
 pH 4.5, 100 min, 50% loss of activity Saccharolobus solfataricus
3.2.1.4 90
-
 pH 4.5, 100 min, 50% residual activity Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 4.5
-
-
 Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 2.7 6.5 pH 2.7: 58% of maximal activity, pH 6.5: 62% of maximal activity. Minimal activity below pH 2.2 and above pH 7.0 Saccharolobus solfataricus
3.2.1.4 2.7
-
 58% residual activity Saccharolobus solfataricus
3.2.1.4 6.5
-
 62% residual activity Saccharolobus solfataricus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 4.5
-
 90°C, 100 min, 50% loss of activity Saccharolobus solfataricus
3.2.1.4 4.5
-
 90°C, 100 min, 50% residual activity Saccharolobus solfataricus

General Information

EC Number General Information Comment Organism
3.2.1.4 physiological function recombinant expression in tobacco leaf. Activity ranges from 92 to 488 nmol 4-methylumbelliferyl-beta-D-cellobioside/min/mg, the morphology and growth behavior of the transgenic plants are not different to those of wild-type plants Saccharolobus solfataricus