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Literature summary extracted from
- Development of a novel fluorescent protein construct by genetically fusing green fluorescent protein to the N-terminal of aspartate dehydrogenase (2013), Biotechnol. Appl. Biochem., 60, 399-404.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.4.1.21 | analysis | development of a genetically encoded fluorescent protein construct for monitoring of L-Asp in vitro, and employment of aspartate dehydrogenase scaffold as a biorecognition element | Thermotoga maritima |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.4.1.21 | expression of N-terminally His-tagged and GFP-tagged enzyme, using the flexible GGSGG linker, in Escherichia coli. The recombinant tagged aspartate dehydrogenase functions as the biorecognition element, and aspartate-induced conformational change is converted to a fluorescence signal by GFP, method, overview | Thermotoga maritima |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.1.21 | L-aspartate + H2O + NAD(P)+ | Thermotoga maritima | - |
oxaloacetate + NH3 + NAD(P)H + H+ | - |
r |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.4.1.21 | Thermotoga maritima | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.4.1.21 | recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography | Thermotoga maritima |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.4.1.21 | L-aspartate + H2O + NAD(P)+ | - |
Thermotoga maritima | oxaloacetate + NH3 + NAD(P)H + H+ | - |
r | |
| 1.4.1.21 | additional information | high substrate specificity of aspartate dehydrogenase enzyme | Thermotoga maritima | ? | - |
? | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.4.1.21 | NAD+ | - |
Thermotoga maritima | |
| 1.4.1.21 | NADH | - |
Thermotoga maritima | |
| 1.4.1.21 | NADP+ | - |
Thermotoga maritima | |
| 1.4.1.21 | NADPH | - |
Thermotoga maritima | |
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Release 2023.1 (January 2023)
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