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Literature summary extracted from

  • Masullo, M.; Raimo, G.; Dello Russo, A.; Bocchini, V.; Bannister, J.V.
    Purification and characterization of NADH oxidase from the archaea Sulfolobus acidocaldarius and Sulfolobus solfataricus (1996), Biotechnol. Appl. Biochem., 23, 47-54.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.6.3.3 Guanidine-HCl
-
 Saccharolobus solfataricus
1.6.3.3 Guanidine-HCl
-
 Sulfolobus acidocaldarius
1.6.3.3 Urea
-
 Saccharolobus solfataricus
1.6.3.3 Urea
-
 Sulfolobus acidocaldarius

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.3.3 27000
-
 1 * 27000 Sulfolobus acidocaldarius
1.6.3.3 35000
-
 2 * 35000 Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.3.3 NADH + H+ + O2 Sulfolobus acidocaldarius
-
 NAD+ + H2O2
-
 ?
1.6.3.3 NADH + H+ + O2 Saccharolobus solfataricus
-
 NAD+ + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.3.3 Saccharolobus solfataricus
-
-
-
1.6.3.3 Sulfolobus acidocaldarius
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.3.3
-
 Sulfolobus acidocaldarius
1.6.3.3
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.3.3 NADH + H+ + O2
-
 Sulfolobus acidocaldarius NAD+ + H2O2
-
 ?
1.6.3.3 NADH + H+ + O2
-
 Saccharolobus solfataricus NAD+ + H2O2
-
 ?
1.6.3.3 NADH + H+ + O2 oxidizes specifically beta-NADH in the presence of molecular oxygen Sulfolobus acidocaldarius NAD+ + H2O2
-
 ?
1.6.3.3 NADH + H+ + O2 oxidizes specifically beta-NADH in the presence of molecular oxygen Saccharolobus solfataricus NAD+ + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.3.3 dimer 2 * 35000 Saccharolobus solfataricus
1.6.3.3 monomer 1 * 27000 Sulfolobus acidocaldarius

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.3.3 90
-
 half-life: 180 min Sulfolobus acidocaldarius
1.6.3.3 98
-
 half-life: 77 min Saccharolobus solfataricus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.3.3 FAD requires the presence of a flavin cofactor, showing a high specificity for FAD. The enzyme does not contain a flavin molecule Sulfolobus acidocaldarius
1.6.3.3 FAD requires the presence of a flavin cofactor, showing a high specificity for FAD. The enzyme is purified as an FAD-containing protein Saccharolobus solfataricus