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Literature summary extracted from

  • Kato, N.; Suzuki, H.; Okumura, H.; Takahashi, S.; Osada, H.
    A point mutation in ftmD blocks the fumitremorgin biosynthetic pathway in Aspergillus fumigatus strain Af293 (2013), Biosci. Biotechnol. Biochem., 77, 1061-1067.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.293 expressed in Escherichia coli BL21(DE3) cells Aspergillus fumigatus

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.293 R202L the mutant has 19.7times lower catalytic efficiency compared to the wild type enzyme Aspergillus fumigatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.293 0.0045
-
 S-adenosyl-L-methionine mutant enzyme R202L, at pH 6.8 and 15°C Aspergillus fumigatus
2.1.1.293 0.011
-
 S-adenosyl-L-methionine wild type enzyme, at pH 6.8 and 15°C Aspergillus fumigatus
2.1.1.293 0.021
-
 6-hydroxytryprostatin B wild type enzyme, at pH 6.8 and 15°C Aspergillus fumigatus
2.1.1.293 0.12
-
 6-hydroxytryprostatin B mutant enzyme R202L, at pH 6.8 and 15°C Aspergillus fumigatus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.293 64000
-
 2 * 64000, SDS-PAGE Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.293 S-adenosyl-L-methionine + 6-hydroxytryprostatin B Aspergillus fumigatus
-
 S-adenosyl-L-homocysteine + tryprostatin A
-
 ?
2.1.1.293 S-adenosyl-L-methionine + 6-hydroxytryprostatin B Aspergillus fumigatus Af293
-
 S-adenosyl-L-homocysteine + tryprostatin A
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.293 Aspergillus fumigatus B9WZX2
-
-
2.1.1.293 Aspergillus fumigatus Af293 B9WZX2
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.293 Talon-Co2+ column chromatography Aspergillus fumigatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.293 S-adenosyl-L-methionine + 6-hydroxytryprostatin B
-
 Aspergillus fumigatus S-adenosyl-L-homocysteine + tryprostatin A
-
 ?
2.1.1.293 S-adenosyl-L-methionine + 6-hydroxytryprostatin B
-
 Aspergillus fumigatus Af293 S-adenosyl-L-homocysteine + tryprostatin A
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.293 homodimer 2 * 64000, SDS-PAGE Aspergillus fumigatus

Synonyms

EC Number Synonyms Comment Organism
2.1.1.293 ftmD
-
 Aspergillus fumigatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.293 15
-
-
 Aspergillus fumigatus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.1.1.293 10 40 the wild type enzyme shows 100% activity at 10°C, about 95% activity at 20°C, about 75% activity at 30°C, about 35% activity at 35°C, and no activity at 40°C Aspergillus fumigatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.293 2 8 S-adenosyl-L-methionine wild type enzyme, at pH 6.8 and 15°C Aspergillus fumigatus
2.1.1.293 6.9
-
 S-adenosyl-L-methionine mutant enzyme R202L, at pH 6.8 and 15°C Aspergillus fumigatus
2.1.1.293 8.2
-
 6-hydroxytryprostatin B mutant enzyme R202L, at pH 6.8 and 15°C Aspergillus fumigatus
2.1.1.293 27
-
 6-hydroxytryprostatin B wild type enzyme, at pH 6.8 and 15°C Aspergillus fumigatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.293 6.8
-
-
 Aspergillus fumigatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.1.1.293 6 7 the wild type enzyme shows about 42% activity at pH 6.0, about 78% activity at pH 6.5, and about 90% activity at pH 7.0 Aspergillus fumigatus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.1.1.293 66
-
 6-hydroxytryprostatin B mutant enzyme R202L, at pH 6.8 and 15°C Aspergillus fumigatus
2.1.1.293 1300
-
 6-hydroxytryprostatin B wild type enzyme, at pH 6.8 and 15°C Aspergillus fumigatus
2.1.1.293 1500
-
 S-adenosyl-L-methionine mutant enzyme R202L, at pH 6.8 and 15°C Aspergillus fumigatus
2.1.1.293 2500
-
 S-adenosyl-L-methionine wild type enzyme, at pH 6.8 and 15°C Aspergillus fumigatus