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Literature summary extracted from

  • Hatta, T.; Fujii, E.; Takizawa, N.
    Analysis of two gene clusters involved in 2,4,6-trichlorophenol degradation by Ralstonia pickettii DTP0602 (2012), Biosci. Biotechnol. Biochem., 76, 892-899.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.32 cloned and expressed in Escherichia coli Ralstonia pickettii

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.32 Ralstonia pickettii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.1.32 purified 433fold using DEAR-Toyopearl column chromatography, phenyl-sepharose column chromatography and Mono Q column chromatography Ralstonia pickettii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.32 2-maleylacetate + NADPH
-
 Ralstonia pickettii 3-oxoadipate + NADP+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.32 hadD
-
 Ralstonia pickettii
1.3.1.32 maleylacetate reductase
-
 Ralstonia pickettii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.1.32 30
-
 assay at Ralstonia pickettii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.1.32 7
-
 assay at Ralstonia pickettii

General Information

EC Number General Information Comment Organism
1.3.1.32 malfunction cells bearing a disrupted hadD gene do not grow on plates with 2,4,6-TCP Ralstonia pickettii
1.3.1.32 physiological function maleylacetate reductase is responsible for 2,4,6-trichlorophenol degradation in Ralstonia pickettii DTP0602 Ralstonia pickettii