EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.41 | 2-mercaptoethanol | slight activation | Burkholderia sp. |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.13.11.41 | gene dad, DNA and amino acid sequence determination and analysis, sequence comparisons, overexpression in Escherichia coli strain JM109 | Burkholderia sp. |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.41 | 1,10-phenanthroline | - |
Burkholderia sp. | |
1.13.11.41 | 2,2'-dipyridine | - |
Burkholderia sp. | |
1.13.11.41 | 4-chloromercuribenzoate | - |
Burkholderia sp. | |
1.13.11.41 | 8-hydroxyquinoline | - |
Burkholderia sp. | |
1.13.11.41 | Co2+ | - |
Burkholderia sp. | |
1.13.11.41 | Cu2+ | strong inhibition | Burkholderia sp. | |
1.13.11.41 | diethyldicarbonate | - |
Burkholderia sp. | |
1.13.11.41 | Hg2+ | strong inhibition | Burkholderia sp. | |
1.13.11.41 | Ni2+ | - |
Burkholderia sp. | |
1.13.11.41 | Tiron | - |
Burkholderia sp. | |
1.13.11.41 | Zn2+ | - |
Burkholderia sp. |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.13.11.41 | 0.0016 | - |
2,4'-dihydroxyacetophenone | pH 7.0, 25°C, recombinant enzyme | Burkholderia sp. |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.13.11.41 | Fe2+ | the enzyme contains 1.69 mol of non-heme iron per mol of enzyme, His77, His79, His115, and Glu96 in the cupin fold are putative metal ligands, Fe2+ weakly activates the enzyme | Burkholderia sp. | |
1.13.11.41 | additional information | poor or no effects by Fe3+, Ca2+ and Mg2+ | Burkholderia sp. |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.13.11.41 | 23000 | - |
4 * 23000, recombinant enzyme, SDS-PAGE | Burkholderia sp. |
1.13.11.41 | 90000 | - |
recombinant enzyme, gel filtration | Burkholderia sp. |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.41 | 2,4'-dihydroxyacetophenone + O2 | Burkholderia sp. | - |
4-hydroxybenzoate + formate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.13.11.41 | Burkholderia sp. | F7J5X4 | isolated from a soil ssampple in Gifu, Japan, gene dad | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.13.11.41 | recombinant enzyme 39fold from Escherichia coli strain JM109 by heat treatment, ammonium sulfate fractionation, gel filtration, and anion exchange chromatography, native enzyme 17fold from strain AZ11 by gel filtration, anion exchange and hydrophobic interaction chromatography | Burkholderia sp. |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.13.11.41 | 0.042 | - |
crude enzyme extract of strain AZ11, pH 7.0, 25°C | Burkholderia sp. |
1.13.11.41 | 0.33 | - |
crude enzyme extract of recombinant Escherichia coli strain JM109 expressing gene dad, pH 7.0, 25°C | Burkholderia sp. |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.13.11.41 | 2,4'-dihydroxyacetophenone + O2 | - |
Burkholderia sp. | 4-hydroxybenzoate + formate | - |
? | |
1.13.11.41 | 2,4'-dihydroxyacetophenone + O2 | anaerobic conditions | Burkholderia sp. | 4-hydroxybenzoate + formate | - |
? | |
1.13.11.41 | additional information | 2-hydroxyacetophenone is a poor substrate | Burkholderia sp. | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.13.11.41 | homotetramer | 4 * 23000, recombinant enzyme, SDS-PAGE | Burkholderia sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.13.11.41 | DAD | - |
Burkholderia sp. |
1.13.11.41 | DHAP dioxygenase | - |
Burkholderia sp. |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.11.41 | 25 | - |
assay at | Burkholderia sp. |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.13.11.41 | 70 | - |
purified enzyme, 20 min, over 80% activity remaining | Burkholderia sp. |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.13.11.41 | 6 | 7 | - |
Burkholderia sp. |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.13.11.41 | metabolism | the enzyme is involved in degradation of bisphenol A | Burkholderia sp. |
1.13.11.41 | physiological function | the enzyme is involved in degradation of bisphenol A | Burkholderia sp. |