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Literature summary extracted from

  • Ohshima, T.
    Structural characteristics of active and inactive glutamate dehydrogenases from the hyperthermophile Pyrobaculum islandicum (2012), Biosci. Biotechnol. Biochem., 76, 1601-1610.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.4.1.2 additional information the enzyme is activated by heat above 70°C, urea and organic solvents, but not by salts. Activation of recombinant enzyme by urea and heat, that is purified as inactive enzyme at 4°C Pyrobaculum islandicum
1.4.1.2 Urea activates the enzyme irreversibly at 5 M Pyrobaculum islandicum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.2 expression of inactive enzyme in Escherichia coli Pyrobaculum islandicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.1.2 47000
-
 6 * 47000, SDS-PAGE Pyrobaculum islandicum
1.4.1.2 280000
-
-
 Pyrobaculum islandicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.2 L-glutamate + H2O + NAD+ Pyrobaculum islandicum
-
 2-oxoglutarate + NH3 + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.2 Pyrobaculum islandicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.2 inactivate recombinant enzyme expressed from Escherichia coli by affinity and anion exchange chromatography, followed by gel filtrration, activation by 5 M urea and 70°c Pyrobaculum islandicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.2 L-glutamate + H2O + NAD+
-
 Pyrobaculum islandicum 2-oxoglutarate + NH3 + NADH + H+
-
 r
1.4.1.2 L-norvaline + H2O + NAD+
-
 Pyrobaculum islandicum 2-oxopentanoate + NH3 + NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.4.1.2 hexamer 6 * 47000, SDS-PAGE Pyrobaculum islandicum
1.4.1.2 More three-dimensional structure and structure-activity relationship, modeling, comparison to other hyperthermophilic enzymes from Pyrococcus furiosus and Thermococcus litoralis, overview Pyrobaculum islandicum

Synonyms

EC Number Synonyms Comment Organism
1.4.1.2 NAD-dependent glutamate dehydrogenase
-
 Pyrobaculum islandicum
1.4.1.2 NAD-GDH
-
 Pyrobaculum islandicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.1.2 90
-
 at pH 9.7 Pyrobaculum islandicum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.1.2 additional information
-
 the enzyme shows extremely high temperature stability, molecular mechanism and comparison to other hyperthermophilic enzymes from Pyrococcus furiosus and Thermococcus litoralis, overview Pyrobaculum islandicum
1.4.1.2 100
-
 pH 7.2, 120 min, stable Pyrobaculum islandicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.1.2 8.5
-
 amination Pyrobaculum islandicum
1.4.1.2 9.7
-
 deamination Pyrobaculum islandicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.2 NAD+
-
 Pyrobaculum islandicum
1.4.1.2 NADH
-
 Pyrobaculum islandicum

General Information

EC Number General Information Comment Organism
1.4.1.2 additional information structure-activity relationship, modeling, comparison to other hyperthermophilic enzymes from Pyrococcus furiosus and Thermococcus litoralis, overview Pyrobaculum islandicum