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Literature summary extracted from

  • Ohmori, T.; Morita, H.; Tanaka, M.; Tomoi, M.; Miyauchi, K.; Kasai, D.; Furukawa, K.; Masai, E.; Fukuda, M.
    Expression in Escherichia coli of biphenyl 2,3-dioxygenase genes from a Gram-positive polychlorinated biphenyl degrader, Rhodococcus jostii RHA1 (2011), Biosci. Biotechnol. Biochem., 75, 26-33.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.18 gene cluster bphAaAbAcAd, genetic organization, the multicomponent enzyme components cannot be expressed in Escherichia coli actively due to lack of activity of the ferredoxin component in Escherichia coli involving codon usage bias. Therefore hybrid BphA gene derivatives are constructed by replacing ferredoxin and/or reductase components of RHA1 with those of Pseudomonas pseudoalcaligenes strain KF707. Expression of ferredoxin encoding gene bphAc in Escherichia coli strain Rosetta (DE3) pLacI resulting in an active protein Rhodococcus jostii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.12.18 Fe2+ one Fe+ and one [Fe2-S2] cluster in the large subunit, as well as one [Fe2-S2] cluster in the small subunit of the enzyme complex Rhodococcus jostii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.18 biphenyl + NADH + H+ + O2 Rhodococcus jostii
-
 (1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.18 Rhodococcus jostii
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.18 biphenyl + NADH + H+ + O2
-
 Rhodococcus jostii (1S,2R)-3-phenylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.18 BphA
-
 Rhodococcus jostii

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.18 FAD in the reductase component of the enzyme complex Rhodococcus jostii
1.14.12.18 NADH
-
 Rhodococcus jostii

General Information

EC Number General Information Comment Organism
1.14.12.18 additional information the multicomponent enzyme consists of small and large subunits of the oxygenase component, and ferredoxin and reductase components Rhodococcus jostii