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Literature summary extracted from

  • Nishimura, M.; Kohno, K.; Nishimura, Y.; Inagaki, M.; Davies, J.
    Characterization of two isozymes of coniferyl alcohol dehydrogenase from Streptomyces sp. NL15-2K (2011), Biosci. Biotechnol. Biochem., 75, 1770-1777.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.90 Fe3+ 1 mM, 30% residual activity Streptomyces sp.
1.1.1.90 iodoacetic acid 1 mM, 57% residual activity Streptomyces sp.
1.1.1.90 N-bromosuccinimide 1 mM, 3% residual activity Streptomyces sp.
1.1.1.90 Zn2+ 1 mM, 9% residual activity Streptomyces sp.
1.1.1.195 Fe3+ 1 mM, 74% residual activity Streptomyces sp.
1.1.1.195 iodoacetic acid 1 mM, 56% residual activity Streptomyces sp.
1.1.1.195 N-bromosuccimide 1 mM, 56% residual activity Streptomyces sp.
1.1.1.195 Zn2+ 1 mM, 80% residual activity Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.90 0.13
-
 coniferyl alcohol pH 8.0, 30°C Streptomyces sp.
1.1.1.90 0.4
-
 NAD+ pH 8.0, 30°C Streptomyces sp.
1.1.1.90 1.7
-
 cinnamyl alcohol pH 8.0, 30°C Streptomyces sp.
1.1.1.90 5.1
-
 coniferyl aldehyde pH 8.0, 30°C Streptomyces sp.
1.1.1.195 0.052
-
 NAD+ pH 8.0, 30°C Streptomyces sp.
1.1.1.195 0.17
-
 cinnamyl alcohol pH 8.0, 30°C Streptomyces sp.
1.1.1.195 0.5
-
 allyl alcohol pH 8.0, 30°C Streptomyces sp.
1.1.1.195 0.92
-
 cinnamaldehyde pH 8.0, 30°C Streptomyces sp.
1.1.1.195 0.96
-
 3-Phenyl-1-propanol pH 8.0, 30°C Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.90 37598
-
 4 * 37598, MALDI-TOF Streptomyces sp.
1.1.1.90 151000
-
 gel filtration Streptomyces sp.
1.1.1.195 35782
-
 4 * 35782, MALDI-TOF Streptomyces sp.
1.1.1.195 143000
-
 gel filtration Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.90 Streptomyces sp. M5AJW4
-
-
1.1.1.195 Streptomyces sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.90
-
 Streptomyces sp.
1.1.1.195
-
 Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.90 cinnamic aldehyde + NADH + H+
-
 Streptomyces sp. cinnamyl alcohol + NAD+
-
 r
1.1.1.90 cinnamyl alcohol + NAD+
-
 Streptomyces sp. cinnamic aldehyde + NADH + H+
-
 r
1.1.1.90 coniferyl alcohol + NAD+
-
 Streptomyces sp. coniferyl aldehyde + NADH + H+
-
 r
1.1.1.90 coniferyl aldehyde + NADH + H+
-
 Streptomyces sp. coniferyl alcohol + NAD+
-
 r
1.1.1.195 3-phenyl-1-propanol + NAD+
-
 Streptomyces sp. 3-phenylpropanal + NADH + H+
-
 r
1.1.1.195 allyl alcohol + NAD+
-
 Streptomyces sp. prop-2-enal + NADH + H+
-
 r
1.1.1.195 cinnamaldehyde + NADH + H+
-
 Streptomyces sp. cinnamyl alcohol + NAD+
-
 r
1.1.1.195 cinnamyl alcohol + NADP+
-
 Streptomyces sp. cinnamaldehyde + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.90 tetramer 4 * 37598, MALDI-TOF Streptomyces sp.
1.1.1.195 tetramer 4 * 35782, MALDI-TOF Streptomyces sp.

Synonyms

EC Number Synonyms Comment Organism
1.1.1.90 CADH II
-
 Streptomyces sp.
1.1.1.195 CADH I
-
 Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.90 40
-
-
 Streptomyces sp.
1.1.1.195 45
-
-
 Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.90 50
-
 pH 7.0, stable up to Streptomyces sp.
1.1.1.195 50
-
 pH 7.0, stable up to Streptomyces sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.90 53.7
-
 NAD+ pH 8.0, 30°C Streptomyces sp.
1.1.1.90 69.7
-
 coniferyl alcohol pH 8.0, 30°C Streptomyces sp.
1.1.1.90 116
-
 cinnamyl alcohol pH 8.0, 30°C Streptomyces sp.
1.1.1.90 354
-
 coniferyl aldehyde pH 8.0, 30°C Streptomyces sp.
1.1.1.195 11.2
-
 3-Phenyl-1-propanol pH 8.0, 30°C Streptomyces sp.
1.1.1.195 14.4
-
 cinnamaldehyde pH 8.0, 30°C Streptomyces sp.
1.1.1.195 18.1
-
 cinnamyl alcohol pH 8.0, 30°C Streptomyces sp.
1.1.1.195 19.9
-
 NAD+ pH 8.0, 30°C Streptomyces sp.
1.1.1.195 32.9
-
 allyl alcohol pH 8.0, 30°C Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.90 8.5
-
-
 Streptomyces sp.
1.1.1.195 9.5
-
-
 Streptomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.90 5 9.5 75 h, more than 80% of initial activity Streptomyces sp.
1.1.1.195 5 9.5 75 h, more than 80% of initial activity Streptomyces sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.90 additional information no cofactor: NADPH Streptomyces sp.
1.1.1.90 NAD+
-
 Streptomyces sp.
1.1.1.90 NADH
-
 Streptomyces sp.
1.1.1.195 additional information NADPH is about 4000fold less effective than NADH Streptomyces sp.
1.1.1.195 NAD+
-
 Streptomyces sp.
1.1.1.195 NADH
-
 Streptomyces sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.90 68.3
-
 cinnamyl alcohol pH 8.0, 30°C Streptomyces sp.
1.1.1.90 69.3
-
 coniferyl aldehyde pH 8.0, 30°C Streptomyces sp.
1.1.1.90 134
-
 NAD+ pH 8.0, 30°C Streptomyces sp.
1.1.1.90 536
-
 coniferyl alcohol pH 8.0, 30°C Streptomyces sp.
1.1.1.195 11.6
-
 3-Phenyl-1-propanol pH 8.0, 30°C Streptomyces sp.
1.1.1.195 15.6
-
 cinnamaldehyde pH 8.0, 30°C Streptomyces sp.
1.1.1.195 66.5
-
 allyl alcohol pH 8.0, 30°C Streptomyces sp.
1.1.1.195 106
-
 cinnamyl alcohol pH 8.0, 30°C Streptomyces sp.
1.1.1.195 387
-
 NAD+ pH 8.0, 30°C Streptomyces sp.