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Literature summary extracted from

  • Noh, K.H.; Son, J.W.; Kim, H.J., Oh, D.K.
    Ginsenoside compound K production from ginseng root extract by a thermostable beta-glycosidase from Sulfolobus solfataricus (2009), Biosci. Biotechnol. Biochem., 73, 316-321.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.B26 synthesis synthesis of ginsenoside K (20-O-beta-D-glucopyranosyl-20(S)-protopanaxadiol) by a recombinant enzyme. 20-O-beta-D-Glucopyranosyl-20(S)-protopanaxadiol has anti-tumor, anti-inflammatory, anti-allergic and hepatoprotective effects Saccharolobus solfataricus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.B26 expression in Escherichia coli Saccharolobus solfataricus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.B26 Saccharolobus solfataricus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.B26 root
-
 Saccharolobus solfataricus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.B26 2-nitrophenyl beta-D-galactoside + H2O
-
 Saccharolobus solfataricus 2-nitrophenol + beta-D-galactose
-
 ?
3.2.1.B26 2-nitrophenyl beta-D-glucoside + H2O
-
 Saccharolobus solfataricus 2-nitrophenol + beta-D-glucose
-
 ?
3.2.1.B26 3beta-[beta-D-glucopyranosyl-(1->2)-beta-D-glucopyranosyloxy]-20-[beta-D-glucopyranosyl-(1->6)-beta-D-glucopyranosyloxy]dammar-24-en-12beta-ol + H2O i.e. ginsenoside Rb1 Saccharolobus solfataricus ginsenoside Rd + D-glucose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-galactoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.B26 4-nitrophenyl beta-D-glucoside + H2O
-
 Saccharolobus solfataricus 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.B26 cellobiose + H2O
-
 Saccharolobus solfataricus 2 beta-D-glucose
-
 ?
3.2.1.B26 gentiobiose + H2O
-
 Saccharolobus solfataricus 2 beta-D-glucose
-
 ?
3.2.1.B26 ginsenoside Rb2 + H2O i.e. 3beta-[beta-D-glucopyranosyl-(1->2)-beta-D glucopyranosyloxy]-20-[alpha-L-arabinopyranosyl-(1->6)-beta-D glucopyranosyloxy]dammar-24-en-12beta-ol Saccharolobus solfataricus ginsenoside Rd + L-arabinopyranose
-
 ?
3.2.1.B26 ginsenoside Rc + H2O i.e. 3beta-[beta-D-glucopyranosyl-(1->2)-beta-D glucopyranosyloxy]-20-[alpha-L-arabinofuranosyl-(1->6)-beta-D glucopyranosyloxy]dammar-24-en-12beta-ol Saccharolobus solfataricus ginsenoside Mc + L-arabinofuranose
-
 ?
3.2.1.B26 ginsenoside Rd + H2O i.e. 3beta-[beta-D-glucopyranosyl-(1->2)-beta-D-glucopyranosyloxy]-20-(beta-D-glucopyranosyloxy)dammar-24-en-12beta-ol Saccharolobus solfataricus ginsenoside K + D-glucose
-
 ?
3.2.1.B26 lactose + H2O
-
 Saccharolobus solfataricus beta-D-glucose + beta-D-galactose
-
 ?
3.2.1.B26 laminaribiose + H2O
-
 Saccharolobus solfataricus 3 beta-D-glucose
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.B26 85
-
 assay at Saccharolobus solfataricus
3.2.1.B26 90
-
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.B26 80 100 80°C: 65% of maximal activity, 100°C: 60% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.B26 70
-
 half-life: 700 h Saccharolobus solfataricus
3.2.1.B26 75
-
 half-life: 100 h Saccharolobus solfataricus
3.2.1.B26 80
-
 half-life: 66 h Saccharolobus solfataricus
3.2.1.B26 85
-
 half-life: 30 h Saccharolobus solfataricus
3.2.1.B26 90
-
 half-life: 1.7 h Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.B26 5.5
-
-
 Saccharolobus solfataricus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.B26 4.5 6.5 pH 4.5: about 80% of maximal activity, pH 6.5: about 70% of maximal activity Saccharolobus solfataricus