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Literature summary extracted from

  • Sangar, S.; Pal, M.; Moon, L.S.; Jolly, R.S.
    A catalase-peroxidase for oxidation of beta-lactams to their (R)-sulfoxides (2012), Biores. Technol., 115, 102-110.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.11.1.21 synthesis biocatalytic method for stereoselective oxidation of beta-lactams, such as penicillin-G, penicillin-V and cephalosporin-G to their (R)-sulfoxides Bacillus pumilus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.21 hydroxylamine 0.05 mM, 80% inhibition of peroxidase activity, 88% inhibition of catalyse activity Bacillus pumilus
1.11.1.21 KCN 0.05 mM, 30% inhibition of peroxidase activity, 22% inhibition of catalyse activity Bacillus pumilus
1.11.1.21 Sodium azide 25 mM, 30% inhibition of peroxidase activity, 100% inhibition of catalyse activity Bacillus pumilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.21 3.82
-
H2O2 catalase activity, pH 7.0, 37°C Bacillus pumilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.21 62000
-
gel filtration Bacillus pumilus
1.11.1.21 62140
-
1 * 62400, SDS-PAGE, 1 * 62140, MALDI-TOF Bacillus pumilus
1.11.1.21 62400
-
1 * 62400, SDS-PAGE, 1 * 62140, MALDI-TOF Bacillus pumilus

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.21 Bacillus pumilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.21
-
Bacillus pumilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.21 743
-
peroxidase activity, pH 7.0, 37°C Bacillus pumilus
1.11.1.21 818
-
catalase activity, pH 7.0, 37°C Bacillus pumilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.21 2 H2O2
-
Bacillus pumilus O2 + 2 H2O
-
?
1.11.1.21 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) + H2O2
-
Bacillus pumilus ? + H2O
-
?
1.11.1.21 penicillin G + H2O2
-
Bacillus pumilus penicillin G (R)-sulfoxide + H2O
-
?

Subunits

EC Number Subunits Comment Organism
1.11.1.21 monomer 1 * 62400, SDS-PAGE, 1 * 62140, MALDI-TOF Bacillus pumilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.21 37
-
-
Bacillus pumilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.21 40
-
sharp decrease in activity above Bacillus pumilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.21 7
-
-
Bacillus pumilus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.11.1.21 6.5 8 more than 75% of maximum activity within Bacillus pumilus

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.21 heme
-
Bacillus pumilus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.11.1.21 1300
-
H2O2 catalase activity, pH 7.0, 37°C Bacillus pumilus