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Literature summary extracted from

  • Telke, A.A.; Ghodake, G.S.; Kalyani, D.C.; Dhanve, R.S.; Govindwar, S.P.
    Biochemical characteristics of a textile dye degrading extracellular laccase from a Bacillus sp. ADR (2011), Biores. Technol., 102, 1752-1756.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 dithiothreitol
-
Bacillus sp. (in: Bacteria)
1.10.3.2 L-cysteine
-
Bacillus sp. (in: Bacteria)
1.10.3.2 Sodium azide
-
Bacillus sp. (in: Bacteria)

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular the enzyme is secreted Bacillus sp. (in: Bacteria)
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ purified laccase contains 2.7 mol/mol of copper Bacillus sp. (in: Bacteria)
1.10.3.2 additional information no activation or inhibition by 1 mM of MgCl2, CaCl2, MnCl2, ZnSO4, or CuSO4 Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 66000
-
x * 66000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Bacillus sp. (in: Bacteria)
-
-
-
1.10.3.2 Bacillus sp. (in: Bacteria) ADR
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 native extracelluar enzyme 56fold by acetone precipitation and anion exchange chromatography Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 3.33
-
purified native extracelluar enzyme, pH 4.0, 40°C Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,6-dimethoxyphenol + O2
-
Bacillus sp. (in: Bacteria) ?
-
?
1.10.3.2 2,6-dimethoxyphenol + O2
-
Bacillus sp. (in: Bacteria) ADR ?
-
?
1.10.3.2 guaiacol + O2
-
Bacillus sp. (in: Bacteria) ?
-
?
1.10.3.2 guaiacol + O2
-
Bacillus sp. (in: Bacteria) ADR ?
-
?
1.10.3.2 hydroquinone + O2
-
Bacillus sp. (in: Bacteria) ?
-
?
1.10.3.2 hydroquinone + O2
-
Bacillus sp. (in: Bacteria) ADR ?
-
?
1.10.3.2 L-DOPA + O2
-
Bacillus sp. (in: Bacteria) ?
-
?
1.10.3.2 L-DOPA + O2
-
Bacillus sp. (in: Bacteria) ADR ?
-
?
1.10.3.2 additional information no activity with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), syringaldazine, catechole, benzidine, 4-cresol, pyrogallol, caffeic acid and tyrosine. Decolorization of structurally different azo dyes using purified laccase: Methyl red, Reactive orange 16, Methyl orange, Sudan IV, Reactive red M5B, Direct brown MR, Reactive red 141, no decolorization of Congo red, Eriochrome black T, and Trypan blue Bacillus sp. (in: Bacteria) ?
-
?
1.10.3.2 additional information no activity with 2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), syringaldazine, catechole, benzidine, 4-cresol, pyrogallol, caffeic acid and tyrosine. Decolorization of structurally different azo dyes using purified laccase: Methyl red, Reactive orange 16, Methyl orange, Sudan IV, Reactive red M5B, Direct brown MR, Reactive red 141, no decolorization of Congo red, Eriochrome black T, and Trypan blue Bacillus sp. (in: Bacteria) ADR ?
-
?
1.10.3.2 o-tolidine + O2 best substrate Bacillus sp. (in: Bacteria) ?
-
?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 66000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 40
-
-
Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 40
-
stable up to Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3
-
with substrate o-tolidine Bacillus sp. (in: Bacteria)
1.10.3.2 4
-
with substrate 2,6-dimethoxyphenol Bacillus sp. (in: Bacteria)
1.10.3.2 5
-
with substrate guaiacol Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 7 9 stable at Bacillus sp. (in: Bacteria)