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Literature summary extracted from

  • Zhang, Q.; Qu, Y.; Zhou, J.; Zhang, X.; Zhou, H.; Ma, Q.; Li, X.
    Optimization of 2,3-dihydroxybiphenyl 1,2-dioxygenase expression and its application for biosensor (2011), Biores. Technol., 102, 10553-10560.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.39 gene bphC, expressionin Escherichia coli strain BL21 (DE3) Dyella ginsengisoli

Protein Variants

EC Number Protein Variants Comment Organism
1.13.11.39 additional information usage of the enzyme for construction of a catechol biosensor, method development involving Plackett–Burman design and response surface methodology, overview. Maximal specific activity of BphC_LA-4 is about 0.58 U/mg with catechol as substrate. the BphC_LA-4 enzyme electrode prepared by SiO2 sol–gel shows good response to all these three catecholic compounds Dyella ginsengisoli

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.39 Dyella ginsengisoli
-
 gene bphC
-
1.13.11.39 Dyella ginsengisoli LA-4
-
 gene bphC
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.39 3-methylcatechol + O2
-
 Dyella ginsengisoli ?
-
 ?
1.13.11.39 3-methylcatechol + O2
-
 Dyella ginsengisoli LA-4 ?
-
 ?
1.13.11.39 4-methylcatechol + O2
-
 Dyella ginsengisoli ?
-
 ?
1.13.11.39 4-methylcatechol + O2
-
 Dyella ginsengisoli LA-4 ?
-
 ?
1.13.11.39 catechol + O2
-
 Dyella ginsengisoli cis,cis-muconate
-
 ?
1.13.11.39 catechol + O2
-
 Dyella ginsengisoli LA-4 cis,cis-muconate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.39 2,3-dihydroxybiphenyl 1,2-dioxygenase
-
 Dyella ginsengisoli
1.13.11.39 BphC
-
 Dyella ginsengisoli
1.13.11.39 BphC_LA-4
-
 Dyella ginsengisoli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.39 20
-
 assay at Dyella ginsengisoli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.39 8
-
 assay at Dyella ginsengisoli

General Information

EC Number General Information Comment Organism
1.13.11.39 additional information analysis of the interaction between BphC_LA-4 and catecholic substrates by homology modeling and molecular docking, model of the BphC_LA-4-catechol complex, overview. BphC_LA-4 presents lower binding affinity towards 4-methylcatechol in comparison with 3-methylcatechol and catechol. A BphC_LA-4 enzyme electrode prepared by SiO2 sol-gel shows good response to all these three catecholic compounds Dyella ginsengisoli