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Literature summary extracted from

  • Liu, X.; Wu, L.; Deng, G.; Chen, G.; Li, N.; Chu, X.; Li, D.
    Comparative studies of acyl-CoA dehydrogenases for monomethyl branched chain substrates in amino acid metabolism (2013), Bioorg. Chem., 47, 1-8.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.8.4 DNA and amino acid sequence determination and comparison, expression in Escherichia coli strain BL21(DE3) Rattus norvegicus

Protein Variants

EC Number Protein Variants Comment Organism
1.3.8.4 E254G site-directed mutagenesis, inactive mutant Rattus norvegicus
1.3.8.4 E254G/G375E site-directed mutagenesis, shows no activity with (S)-2-methylbutyryl-CoA in contrast to the wild-type enzyme, reduced activity compared to the wild-type enzyme Rattus norvegicus
1.3.8.4 G375E site-directed mutagenesis, reduced activity compared to the wild-type enzyme Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.8.4 0.0019
-
 (S)-2-methylbutyryl-CoA wild-type enzyme, pH 7.4, 37°C Rattus norvegicus
1.3.8.4 0.0029
-
 isovaleryl-CoA wild-type enzyme, pH 7.4, 37°C Rattus norvegicus
1.3.8.4 0.0031
-
 isovaleryl-CoA mutabt IVD E254G/G375E, pH 7.4, 37°C Rattus norvegicus
1.3.8.4 0.0035
-
 (S)-2-methylbutyryl-CoA mutant IVD G375E, pH 7.4, 37°C Rattus norvegicus
1.3.8.4 0.0038
-
 isovaleryl-CoA mutant IVD G375E, pH 7.4, 37°C Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.8.4 mitochondrion
-
 Rattus norvegicus 5739
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.8.4 isovaleryl-CoA + electron-transfer flavoprotein Rattus norvegicus
-
 3-methylcrotonyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.4 additional information Rattus norvegicus the enzyme catalyzes the dehydrogenation of monomethyl branched-chain fatty acid thioester derivatives ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.8.4 Rattus norvegicus P12007
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.8.4 (S)-2-methylbutyryl-CoA + electron-transfer flavoprotein
-
 Rattus norvegicus 2-methylcrotonyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.4 butyryl-CoA + electron-transfer flavoprotein
-
 Rattus norvegicus crotonyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.4 hexanoyl-CoA + electron-transfer flavoprotein
-
 Rattus norvegicus hex-2-enoyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.4 isovaleryl-CoA + electron-transfer flavoprotein
-
 Rattus norvegicus 3-methylcrotonyl-CoA + reduced electron-transfer flavoprotein
-
 ?
1.3.8.4 additional information the enzyme catalyzes the dehydrogenation of monomethyl branched-chain fatty acid thioester derivatives Rattus norvegicus ?
-
 ?
1.3.8.4 additional information broader substrate specificity, overview. The enzyme is also active with octanoyl-CoA, decanoyl-CoA, and dodecanoyl-CoA. No activity with isobutyryl-CoA and myristoyl-CoA, with DCIPIP and phenazine methosulfate as second electron acceptors Rattus norvegicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.8.4 IVD
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.3.8.4 37
-
 assay at Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.8.4 0.17
-
 isovaleryl-CoA mutant IVD G375E, pH 7.4, 37°C Rattus norvegicus
1.3.8.4 0.3
-
 (S)-2-methylbutyryl-CoA wild-type enzyme and mutant IVD G375E, pH 7.4, 37°C Rattus norvegicus
1.3.8.4 0.32
-
 isovaleryl-CoA mutabt IVD E254G/G375E, pH 7.4, 37°C Rattus norvegicus
1.3.8.4 1.5
-
 isovaleryl-CoA wild-type enzyme, pH 7.4, 37°C Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.8.4 7.4
-
 assay at Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.8.4 electron transferring flavoprotein
-
 Rattus norvegicus

General Information

EC Number General Information Comment Organism
1.3.8.4 evolution the enzyme belongs to acyl-CoA dehydrogenase (ACD) family Rattus norvegicus

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3.8.4 0.045
-
 isovaleryl-CoA mutant IVD G375E, pH 7.4, 37°C Rattus norvegicus
1.3.8.4 0.086
-
 (S)-2-methylbutyryl-CoA mutant IVD G375E, pH 7.4, 37°C Rattus norvegicus
1.3.8.4 0.1
-
 isovaleryl-CoA mutabt IVD E254G/G375E, pH 7.4, 37°C Rattus norvegicus
1.3.8.4 0.16
-
 (S)-2-methylbutyryl-CoA wild-type enzyme, pH 7.4, 37°C Rattus norvegicus
1.3.8.4 0.53
-
 isovaleryl-CoA wild-type enzyme, pH 7.4, 37°C Rattus norvegicus