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Literature summary extracted from

  • Chen, H.S.; Wang, Y.M.; Huang, W.T.; Huang, K.F.; Tsai, I.H.
    Cloning, characterization and mutagenesis of Russells viper venom L-amino acid oxidase: Insights into its catalytic mechanism (2012), Biochimie, 94, 335-344.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.2 expressed in Escherichia coli JM109 cells and in Pichia pastoris strain KM71H Daboia russelii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.2 0.081
-
 L-Tyr pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
1.4.3.2 0.142
-
 L-Phe pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
1.4.3.2 0.318
-
 L-Trp pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
1.4.3.2 0.373
-
 L-Met pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
1.4.3.2 0.49
-
 L-Leu pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
1.4.3.2 1.4
-
 L-Ile pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
1.4.3.2 12.2
-
 L-Arg pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
1.4.3.2 13.92
-
 L-Val pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
1.4.3.2 64
-
 L-Lys pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
1.4.3.2 116.5
-
 L-Ala pH 7.5, 0.9% NaCl, at 25°C Daboia russelii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.2 54864
-
 x * 54864, calculated from amino acid sequence Daboia russelii
1.4.3.2 62025
-
 x * 62025, MALDI-TOF mass spectrometry Daboia russelii
1.4.3.2 63600
-
 x * 63600, SDS-PAGE Daboia russelii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.2 L-amino acid + H2O + O2 Daboia russelii
-
 a 2-oxo carboxylate + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Daboia russelii
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.4.3.2 glycoprotein the enzyme has three potential glycosylation sites at Asn 172, 194, and 361 Daboia russelii

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.2 ammonium sulfate precipitation, Mono Q column chromatography, and heparin-Sepharose column chromatography Daboia russelii

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.2 venom
-
 Daboia russelii
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 L-Ala + H2O + O2 the substrate is not effectively oxidized Daboia russelii 2-oxopropanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-amino acid + H2O + O2
-
 Daboia russelii a 2-oxo carboxylate + NH3 + H2O2
-
 ?
1.4.3.2 L-Arg + H2O + O2 the substrate is not effectively oxidized Daboia russelii 5-carbamidamido-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Ile + H2O + O2
-
 Daboia russelii 3-methyl-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Leu + H2O + O2 best substrate Daboia russelii 4-methyl-2-oxopentanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Lys + H2O + O2 the substrate is not effectively oxidized Daboia russelii 6-amino-2-oxohexanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Met + H2O + O2
-
 Daboia russelii 4-methylthio-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Phe + H2O + O2
-
 Daboia russelii 2-oxo-3-phenylpropanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Trp + H2O + O2
-
 Daboia russelii 3-(1H-indol-3-yl)-2-oxopropanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Tyr + H2O + O2
-
 Daboia russelii 3-(4-hydroxyphenyl)-2-oxopropanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Val + H2O + O2 the substrate is not effectively oxidized Daboia russelii 3-methyl-2-oxobutanoate + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.2 ? x * 54864, calculated from amino acid sequence Daboia russelii
1.4.3.2 ? x * 62025, MALDI-TOF mass spectrometry Daboia russelii
1.4.3.2 ? x * 63600, SDS-PAGE Daboia russelii

Synonyms

EC Number Synonyms Comment Organism
1.4.3.2 L-amino acid oxidase
-
 Daboia russelii
1.4.3.2 LAAO
-
 Daboia russelii

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.3.2 FAD
-
 Daboia russelii

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.4.3.2 Daboia russelii calculated from amino acid sequence
-
 8.6

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.3.2 0.02
-
 L-Ala pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
1.4.3.2 0.03
-
 L-Lys pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
1.4.3.2 0.11
-
 L-Val pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
1.4.3.2 0.94
-
 L-Arg pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
1.4.3.2 3.72
-
 L-Ile pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
1.4.3.2 18.02
-
 L-Trp pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
1.4.3.2 21.53
-
 L-Leu pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
1.4.3.2 21.93
-
 L-Met pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
1.4.3.2 40.56
-
 L-Tyr pH 7.5, 0.9% NaCl, at 25°C Daboia russelii
1.4.3.2 41.3
-
 L-Phe pH 7.5, 0.9% NaCl, at 25°C Daboia russelii