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Literature summary extracted from

  • Giuliani, M.C.; Jourlin-Castelli, C.; Leroy, G.; Hachani, A.; Giudici-Orticoni, M.T.
    Characterization of a new periplasmic single-domain rhodanese encoded by a sulfur-regulated gene in a hyperthermophilic bacterium Aquifex aeolicus (2010), Biochimie, 92, 388-397.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.1.1 iodoacetamide complete inhibition at 20 mM Aquifex aeolicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8.1.1 1
-
 thiosulfate apparent value, in 100 mM Tris-HCl, pH 9.0, 10 mM KCN, at 85°C Aquifex aeolicus
2.8.1.1 1.7
-
 cyanide apparent value, in 100 mM Tris-HCl, pH 9.0, 10 mM KCN, at 85°C Aquifex aeolicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.8.1.1 periplasm
-
 Aquifex aeolicus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.1.1 15000
-
 4 * 15000, calculated from amino acid sequence Aquifex aeolicus
2.8.1.1 18000
-
 4 * 18000, SDS-PAGE Aquifex aeolicus
2.8.1.1 65000
-
 SDS-PAGE Aquifex aeolicus

Organism

EC Number Organism UniProt Comment Textmining
2.8.1.1 Aquifex aeolicus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.1.1 additional information no activity with mercaptopyruvate as sulfur donor Aquifex aeolicus ?
-
 ?
2.8.1.1 thiosulfate + cyanide
-
 Aquifex aeolicus sulfite + thiocyanate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.1.1 homotetramer 4 * 18000, SDS-PAGE Aquifex aeolicus
2.8.1.1 homotetramer 4 * 15000, calculated from amino acid sequence Aquifex aeolicus

Synonyms

EC Number Synonyms Comment Organism
2.8.1.1 Aq-1599
-
 Aquifex aeolicus
2.8.1.1 RhdB2
-
 Aquifex aeolicus
2.8.1.1 rhodanese
-
 Aquifex aeolicus
2.8.1.1 thiosulfate cyanide sulfurtransferase
-
 Aquifex aeolicus
2.8.1.1 thiosulfate:cyanide transferase
-
 Aquifex aeolicus
2.8.1.1 TST
-
 Aquifex aeolicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.1.1 400
-
 thiosulfate in 100 mM Tris-HCl, pH 9.0, 10 mM KCN, at 85°C Aquifex aeolicus

Expression

EC Number Organism Comment Expression
2.8.1.1 Aquifex aeolicus the expression of the aq-1599 gene is induced in the presence of elemental sulfur up